Anaerobic culture medium preparation system

Abstract

The invention discloses an anaerobic culture medium preparation system. The system structurally comprises a water level sensor, gas outlets, brackets, a gas inlet structure, a culture tank and a base,the culture tank is mounted in the middle of the base, the brackets are vertically mounted on two sides of the culture tank, the water level sensor is mounted in the middle of the top of the culturetank, the gas outlets are formed in the culture tank on two sides of the water level sensor, and the gas inlet structure is mounted at the side top of the culture tank. A gas inlet pipe goes deep intothe bottom of a culture medium, and the filled gas is inert gas and does not dissolve in the culture medium, so that the gas floats upwards from the culture medium, and that the filled gas is free from residue is further ensured. The gas inlet pipe and the gas outlets are not located on the same horizontal plane, the gas outlets are located at the top end, the gas inlet pipe is located on the side face, gas enters the culture medium from the bottom, internal gas is completely replaced, and the construction speed of the anaerobic environment is high.

Description

technical field [0001] The invention relates to the field of culture medium, in particular to an anaerobic culture medium preparation system. Background technique [0002] The cultivation of some microorganisms needs to be cultivated in an anaerobic environment, which is mostly used in laboratory cultivation, and the methods of anaerobic environment are: first, use a vacuum pump to evacuate it and then fill it with anaerobic gas, and repeat 2-3 times to achieve sterilization and anaerobic. The second is to use boiling to expel oxygen, which is to put the prepared medium in a triangular flask, heat and boil, and at the same time pass anaerobic gas, keep it for 15-30 minutes, and then Cool to room temperature at the same time, and then pack and sterilize. The third is to vacuumize the anaerobic box and then fill it with anaerobic gas. Place the medium in an anaerobic environment for a period of time to form an anaerobic environment, but then fill it When entering anaerobic ga...

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Problems solved by technology

[0002] The cultivation of some microorganisms needs to be cultivated in an anaerobic environment, which is mostly used in laboratory cultivation, and the methods of anaerobic environment are: first, use a vacuum pump to evacuate it and then fill it with anaerobic gas, and repeat 2-3 times to achieve sterilization and anaerobic. The second is to use boiling to expel oxygen, which is to put the prepared medium in a triangular flask, heat and boil, and at the same time pass anaerobic gas, keep it for 15-30 minutes, and then Cool to room temperature at the same time, and then pack and sterilize. The third is to vacuumize the anaerobic box and then fill it with anaerobic gas. Place the medium in an anaerobic environment for a period of time to form an anaerobic environment, but then fill it When entering anaerobic gas, because the connection structure is easy to leak, the gas entering may not be all anaerobic gas. Secondly, the gas outlet and the inlet are on the same horizontal plane, and the gas at the bottom cannot be replaced, and there is still residue. lead to an anaerobic environment at a slower rate

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