Application of CXC chemokine receptor 4 gene activator in promoting in-vitro proliferation of mesenchymal stem cells

A technology of chemokine receptors and mesenchymal stem cells, applied in the field of stem cells, can solve the problems of limited proliferation speed of bone marrow mesenchymal stem cells, insufficient number of cells, research and application, etc.

Inactive Publication Date: 2020-09-08
宁夏厚泽生物医药科技有限公司
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Problems solved by technology

[0004] However, the in vitro proliferation rate of bone marrow mesenchymal stem cells i

Method used

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  • Application of CXC chemokine receptor 4 gene activator in promoting in-vitro proliferation of mesenchymal stem cells
  • Application of CXC chemokine receptor 4 gene activator in promoting in-vitro proliferation of mesenchymal stem cells

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Embodiment Construction

[0025] The substantive content of the present invention will be described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0026] 1. Experimental materials

[0027] Human bone marrow mesenchymal stem cells were purchased from Shanghai Yansheng Industrial Co., Ltd.

[0028] Fetal bovine serum was purchased from Gibco, and L-DMEM was purchased from ThermoFisher Scientific.

[0029] The purity of cyclic (S-pro-R-leu) dipeptide is not less than 98%.

[0030] The brands of mesenchymal stem cell osteogenic differentiation medium (ready-to-use) and mesenchymal stem cell adipogenic differentiation medium (ready-to-use) are PromoCell, and the product numbers are C-28013 and C-28011, respectively.

[0031] 2. Experimental method

[0032] 1. Cell culture

[0033] Take the recovered human bone marrow mesenchymal stem cells, when the cell growth and confluence reaches 90%, discard the original medium, wash ...

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Abstract

The invention discloses an application of a CXC chemokine receptor 4 gene activator in promoting in-vitro proliferation of mesenchymal stem cells. It is found that cyclo-(4-hydroxy-Pro-Leu)dipeptide has an effect of promoting in-vitro proliferation of bone marrow mesenchymal stem cells, and after intervention culture of the cyclo-(4-hydroxy-Pro-Leu)dipeptide, the bone marrow mesenchymal stem cellsstill have multidirectional differentiation capacity and can be used for seed cells in stem cell engineering. The cyclo-(4-hydroxy-Pro-Leu)dipeptide can exert the effects by activating expression ofCXCR4 in the bone marrow mesenchymal stem cells, and thus the cyclo-(4-hydroxy-Pro-Leu)dipeptide is an effective CXCR4 activator.

Description

technical field [0001] The invention belongs to the field of stem cells and relates to the in vitro proliferation of mesenchymal stem cells, in particular to the application of a CXC chemokine receptor 4 gene activator in promoting the in vitro proliferation of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are a kind of stem cells derived from mesoderm, which have immune regulation function, self-renewal and proliferation, and can differentiate into osteogenic and adipogenic and other somatic cells. Human mesenchymal stem cells have been widely used clinically and have played a significant role in the treatment of various diseases. [0003] Bone marrow mesenchymal stem cells can be induced to differentiate into osteoblasts, nerve cells, adipocytes, etc., and have strong proliferation, multiple differentiation potentials, and homing characteristics. It is relatively safe for clinical treatment, and the therapeutic ...

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2501/998
Inventor 陈茜李敏许泽
Owner 宁夏厚泽生物医药科技有限公司
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