A method for strengthening the film-forming ability of aerobic denitrifying bacteria
An aerobic denitrifying bacteria and film-forming technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problem of poor anti-ZnONPs effect, weak film-forming ability of aerobic denitrifying bacteria, etc. problems, to achieve the effect of increased film-forming ability
Active Publication Date: 2022-07-08
CHONGQING TECH & BUSINESS UNIV +2
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Problems solved by technology
[0004] The purpose of the present invention is to provide a method for strengthening the film-forming ability of aerobic denitrifying bacteria, to solve the problem of weak film-forming ability of aerobic denitrifying bacteria and poor anti-ZnO NPs effect
Method used
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Embodiment 1
[0031] 1 mL of wild-type and enhanced bacterial solutions were respectively added to HM medium with ZnO NPs concentration of 0 mM for continuous cultivation, and the film-forming ability of HNR strains was recorded.
Embodiment 2
[0033] 1 mL of wild-type and enhanced bacterial solutions were respectively added to HM medium with ZnO NPs concentration of 0.25 mM for continuous cultivation, and the film-forming ability of HNR strains was recorded.
Embodiment 3
[0035] 1 mL of wild-type and enhanced bacterial solutions were respectively added to HM medium with ZnO NPs concentration of 0.50 mM for continuous cultivation, and the film-forming ability of HNR strains was recorded.
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The invention belongs to the technical field of biological wastewater treatment, and specifically discloses a method for strengthening the film-forming ability of aerobic denitrifying bacteria. A method for strengthening the film-forming ability of aerobic denitrifying bacteria, comprising: step 1, preparing HM medium and LB medium; step 2, adding a ZnO NPs solution with a concentration of 1.0-1.5mM to the HM medium in step 1 ; Step 3, put the aerobic denitrifying bacteria strain into the HM medium in step 2 for cultivation; Step 4, select and complete the HM medium in step 3, and add it to the LB medium for rejuvenation; Step 5, will complete The aerobic denitrifying bacteria strain after step 4 is added to the HM medium in step 1 for continuous culture. The purpose of the present invention is to provide a method for strengthening the film-forming ability of aerobic denitrifying bacteria, so as to solve the problems of weak film-forming ability of aerobic denitrifying bacteria and poor anti-ZnO NPs effect.
Description
technical field [0001] The invention belongs to the technical field of biological wastewater treatment, and specifically discloses a method for strengthening the film-forming ability of aerobic denitrifying bacteria. Background technique [0002] At present, with the rapid development of the national economy and the continuous deepening of the urbanization process, the total discharge of urban domestic and industrial wastewater is also increasing year by year. The widespread use of pesticides, chemical fertilizers, and synthetic detergents has led to a continuous increase in the concentration of nutrients in water bodies, among which nitrogen is one of the main causes of eutrophication in water bodies. The conventional denitrification process generally separates the nitrification process and the denitrification process in two reaction units, because most denitrifying bacteria require anaerobic conditions, while nitrifying bacteria require an aerobic environment. The discove...
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IPC IPC(8): C12N1/20C12N1/36C12R1/01
CPCC12N1/20C12N1/36Y02W10/10
Inventor 马腾飞张玮曼尼蒂安·诺帕顿柴旺萨·苏拉风申渝王建辉周月明齐高相
Owner CHONGQING TECH & BUSINESS UNIV

