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Method for detecting correlation between gene GARNL1 and cockscomb and wattle traits of cock and application

A detection method and correlation technology, applied in the field of genetic engineering, to achieve the effect of accurate results, low cost and easy operation

Active Publication Date: 2020-09-11
NANCHANG NORMAL UNIV
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Problems solved by technology

Shen et al. (2012) found that the rs16492031 locus was extremely significantly correlated with the hen's first laying age (P<0.01), but whether this locus is related to the cockscomb and wattle traits of roosters has not been reported

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  • Method for detecting correlation between gene GARNL1 and cockscomb and wattle traits of cock and application
  • Method for detecting correlation between gene GARNL1 and cockscomb and wattle traits of cock and application
  • Method for detecting correlation between gene GARNL1 and cockscomb and wattle traits of cock and application

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Embodiment Construction

[0020] Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention.

[0021] It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention. In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range. ...

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Abstract

The invention discloses a method for detecting correlation between a gene GARNL1 and cockscomb and wattle traits of a cock and application and belongs to the field of genetic engineering. The method comprises the steps of firstly, extracting genomic DNA from a chicken species to be detected, then, amplifying 752bp fragments of upstream and downstream of a locus rs16492031 in an intron 32 of the gene GARNL1, then, subtyping an obtained PCR product by using RFLP, and then, carrying out statistical analysis on correlation between different genotypes of the locus rs16492031 of the gene GARNL1 andthe cockscomb and wattle traits of the cock. According to the application, a relationship for detecting correlation between polymorphism of the locus rs16492031 in the intron of the gene GARNL1 of thechicken species and the cockscomb and wattle traits of the cock is employed, and this relationship and the method serve as means for breeding and marking chicken species. Compared with the present generation breeding, the breeding method has the advantages that the cost is low, the operation is simple and convenient, and the result is accurate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a detection method and application of the correlation between GARNL1 gene and rooster comb and wattle traits. Background technique [0002] In domestic chicken breeding, the traits of precocity are mainly selected from the age at first production, the development of comb and wattle. Zhang Dexiang (1999) researched that it is feasible to select the early maturity of high-quality chickens with the comb area as the index. Wei Fengying et al. (2002) used comb area as the main indicator to carry out early-maturity trait selection in Yinxiang Ma chickens, and found that the breeding age of roosters after 5 generations was advanced by 23 days, and the age of female hens was advanced by 15 days. 400 Day-old egg production increased by 20 eggs. Ji Congliang et al. (2008) found that there is a correlation between early comb height and sexual maturity, and there is a correlation between...

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 周敏谭玉文朱学农许继国贡继尚文正亚
Owner NANCHANG NORMAL UNIV
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