A biological drying and stabilizing bacteria agent for kitchen waste, its preparation method and application

A microbial strain, microorganism technology, applied in the direction of biochemical equipment and methods, microorganism-based methods, microorganisms, etc.

Active Publication Date: 2022-06-14
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there has not been any research on the targeted biological drying of kitchen waste using microbial complex agents

Method used

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  • A biological drying and stabilizing bacteria agent for kitchen waste, its preparation method and application
  • A biological drying and stabilizing bacteria agent for kitchen waste, its preparation method and application
  • A biological drying and stabilizing bacteria agent for kitchen waste, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] 2. The present invention also provides a method for preparing a microbial composite bacterial agent that can specifically treat kitchen waste. Specifically include the following steps:

[0035] 1. Cultivate the starch-degrading bacteria, protein-degrading bacteria, cellulose-degrading bacteria, oil-degrading bacteria and deodorizing bacteria respectively.

[0036] 2. Mix the cultured starch-degrading bacteria, protein-degrading bacteria, cellulose-degrading bacteria, oil-degrading bacteria, and deodorizing bacteria according to the above ratio to obtain composite bacteria.

[0037] 3. Add the composite bacteria into the solid fermentation auxiliary material, and ferment for 3 to 7 days in the range of 55% to 65% humidity to obtain the required microbial composite bacteria agent.

[0038] Three, the present invention also provides a kind of application method of described microbial composite bacterial agent. Specifically include the following steps:

[0039] 1. Put th...

Embodiment 1

[0051] Embodiment one: the screening of each microorganism

[0052] This example briefly demonstrates the screening process of each microorganism in the microbial composite bacterial agent. It should be noted that the inventor actually did not only carry out the following tests; but carried out a large number of screening and optimization tests, and repeatedly verified whether there was antagonism and synergy before finally obtaining and selecting each of the microbial compound bacterial agents. microorganism. Due to space reasons, only a small number of representative works performed by the inventors are selected for presentation in this embodiment.

[0053] The microorganisms in the microbial composite bacterial agent cannot be replaced randomly among the same species; after the replacement, the treatment effect on kitchen waste may be reduced, and even due to antagonism, etc., the kitchen waste cannot be effectively treated, and the purpose of the invention cannot be achie...

Embodiment 2

[0092] Example 2: Preparation and effect display of microbial compound bacterial agent

[0093] 1. Expand cultivation.

[0094] The above-mentioned primary seed solutions of starch-degrading bacteria H5, protein-degrading bacteria N10 and 50-11, cellulose-degrading bacteria ZJ3, oil-degrading bacteria M4 and H7, and deodorizing bacteria DS-4 and SD2-2 were prepared respectively. In each level of seed liquid, the amount of live bacteria is ≥10 9 CFU / mL.

[0095] Under aseptic conditions, pick one loop from the primary seed solution of starch-degrading bacteria H5, protein-degrading bacteria N10 and 50-11, cellulose-degrading bacteria ZJ3, oil-degrading bacteria M4 and H7, and inoculate them in 8 containers. In a 250mL Erlenmeyer flask with 50mL beef extract peptone liquid medium or PDA liquid medium (ZJ3 was inoculated in PDA liquid medium, and the rest of the microorganisms were inoculated in beef extract peptone liquid medium), according to the suitable temperature of each ...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a bio-drying and stabilizing bacterial agent for kitchen waste and its preparation method and application. The specific technical scheme is: a strain of Pseudomonas aeruginosa is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No.17868. And using the Pseudomonas aeruginosa to provide a microbial composition, including: starch-degrading bacteria, protein-degrading bacteria, cellulose-degrading bacteria, oil-degrading bacteria and deodorizing bacteria; the oil-degrading bacteria include Pseudomonas aeruginosa. The microbial composition can treat kitchen waste in a targeted manner, and realize the purposes of dehydration and drying of kitchen waste, volume reduction and resource utilization.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bio-drying and stabilizing bacterial agent for kitchen waste and its preparation method and application. Background technique [0002] Kitchen waste accounts for more than 60% of domestic waste; its high organic matter content is very easy to spoil and deteriorate, and it is the main source of urban life odor pollution. Therefore, how to recycle, reduce, and harmlessly treat kitchen waste is an important problem that needs to be solved urgently. [0003] In the prior art, it is one of the important means to solve the above-mentioned problems to mix the kitchen waste with other domestic waste and then incinerate and generate electricity. However, the moisture content of domestic waste is about 50%, while the moisture content of kitchen waste is as high as 80%. This leads to high water content, low calorific value, and extremely poor combustibility after the two ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F9/04C05F17/20C12R1/385C12R1/01C12R1/07C12R1/645
CPCC12N1/20C05F9/04C05F17/20C12R2001/385C12N1/205C12R2001/07C12R2001/01C12R2001/645C12N1/145Y02A40/20
Inventor 李东石娟
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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