Multi-mode microscopic image numerical reconstruction method

Abstract

The invention discloses a multi-mode microscopic image numerical reconstruction method, which comprises the following steps of: firstly, illuminating a sample by using K different structured lights, and sequentially shooting Fourier spectrum bright spots of light waves of a sample object to obtain K digital Fourier spectrum images; secondly, encoding each Fourier spectrum image by using the digital mask plate matrix to obtain a corresponding encoded spectrum image matrix; summing all matrix element integrals of each coded spectrum image matrix to obtain K Fourier spectrum integral values; carrying out image reconstruction on the K Fourier spectrum integral values, and a digital image obtained through reconstruction being a microscopic image of one mode; and finally, carrying out numericalreconstruction of microscopic images in different modes by using the Fourier spectrum image, and finally, obtaining microscopic images in multiple modes and differential phase contrast microscopic images in one or more modes. According to the method, microscopic images in various different modes can be obtained very flexibly through numerical reconstruction, and the method has important application value.

Description

technical field [0001] The invention relates to the technical field of optical imaging, in particular to a multi-mode microscopic image numerical reconstruction method. Background technique [0002] Microscopic images of samples obtained under different microscopic imaging modes (such as bright field, dark field, ring illumination and differential phase contrast) have their own characteristics and can provide different information about the sample. The traditional microscopic imaging method usually illuminates the sample evenly with the light beam, and directly collects the image on the image plane of the light wave of the sample object. However, in order to observe multi-mode microscopic images such as bright field, dark field, ring illumination and differential phase contrast of the same sample in this existing microscopic imaging method, it is usually necessary to replace different microscope parts and carry out mechanical adjustments. Movement, adjustment, and even need...

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Problems solved by technology

However, in order to observe multi-mode microscopic images such as bright field, dark field, ring illumination and differential phase contrast of the same sample in this existing microscopic imaging method, it is usually necessary to replace different microscope parts and carry out mechanical adjustments. Movement, adjustment, and even need to be carried out under different microscopes, resulting in cumbersome operations, and it is difficult to observe the same field of view of the same sample under different imaging modes

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