Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Screening and application of efficient straw cellulose decomposition bacteria

A cellulolytic bacteria, straw cellulose technology, applied in the application, bacteria, analytical materials and other directions, can solve problems such as the inability to meet large-scale industrial production, achieve good results, enhance palatability, improve reducing sugar content and protein content Effect

Pending Publication Date: 2020-09-22
ANHUI AGRICULTURAL UNIVERSITY +1
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The premise of this application prospect is to first isolate microbial strains that can effectively decompose cellulose, but the cellulase obtained at present, whether it is from animals, plants or microorganisms, cannot meet the needs of large-scale industrial production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening and application of efficient straw cellulose decomposition bacteria
  • Screening and application of efficient straw cellulose decomposition bacteria
  • Screening and application of efficient straw cellulose decomposition bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The screening of efficient straw cellulolytic bacteria comprises the following steps:

[0078] Primary screening of bacterial strains, as mentioned above, primary screening of cellulolytic bacteria: Take 0.1 mL of the sample after homogenization and filtration, put it into 100 mL enrichment medium, and culture at 37°C for 2-3 days. Take 100 μL of the enriched culture solution for gradient (10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 ) dilution; take 10 -4 、10 -5 、10 -6 Spread 100 μL of the diluted bacterial solution on a sodium carboxymethyl cellulose plate, and incubate it upside down at 37°C for 24-48 hours; count the colonies after the bacteria grow out, and then select the colonies with obvious differences in colony morphology, and inoculate them repeatedly by streaking on the carboxymethyl cellulose plate. Cellulose-based sodium agar plate until a single colony was obtained after purification, then transferred to the corresponding agar slant, and stored at 4°C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses screening of efficient straw cellulose decomposition bacteria. Screening of the efficient straw cellulose decomposition bacteria comprises the following steps: (1) conducting preliminary screening on the cellulose decomposition bacteria by adopting a sodium carboxymethylcellulose plate method, and preliminarily measuring the cellulose decomposition capability of the strainsthrough a Congo red staining method and filter paper decomposition rates; (2) measuring the filter paper enzyme activity and the carboxymethyl cellulose enzyme activity of the strains subjected to preliminary screening to continue judging the cellulose decomposition capability; and (3) identifying the cellulose decomposition bacteria, and optimizing enzyme-producing conditions for the strains which highly produce cellulase. According to screening of the efficient straw cellulose decomposition bacteria, the cellulose decomposition bacteria are separated from Dabieshan yellow cattle rumen fluid, are free from bio-safety problems, and have a good effect of fermenting corn straw; through separation and screening in a laboratory, the obtained decomposition bacteria can resist different temperatures and acid and alkali environments with high enzyme activity maintained; and clinical experiments prove that the cellulose decomposition bacteria can effectively promote fermentation of the corn straw, improves a reducing sugar content and a protein content, enhances the palatability, and is easy to digest and absorb.

Description

Technical field: [0001] The invention relates to the field of biology, in particular to the screening and application of high-efficiency straw cellulolytic bacteria. Background technique: [0002] With the rapid development of my country's animal husbandry, although great achievements have been made, it is facing the situation of human and livestock competing for food, which makes the food problem severe. If the structure of animal husbandry is not adjusted in time to reduce the dependence of animal husbandry on grain, the future development will be greatly restricted. At present, there are many research reports on using crop stalks as feed to solve the problem of livestock grain. [0003] The use of microbial technology to treat crop straw is currently the most researched method of straw treatment. A type of cellulolytic bacteria in nature can decompose cellulose such as straw into sugars, and at the same time, the bacteria itself is a very good protein, that is, single-c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/20C12Q1/04C12Q1/34C12Q1/54A23K10/12A23K50/10C12R1/01
CPCC12N1/02C12N1/20C12Q1/04C12Q1/34C12Q1/54A23K10/12A23K50/10G01N2333/942
Inventor 王希春冉梦冯士彬杨长根李玉汤继顺吴金节
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com