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Conductive multifunctional nanoprobe and preparation method and use thereof

A nano-probe and multi-functional technology, applied in the direction of material electrochemical variables, material analysis by electromagnetic means, instruments, etc., can solve the problems of poor conductivity, low loading of signal substances, low catalytic activity, etc., and achieve improved catalytic activity , good electrical conductivity, and the effect of increasing the signal load

Active Publication Date: 2022-08-09
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current common electrochemical immunoprobes have their own shortcomings, such as: poor conductivity, low catalytic activity, low loading of signal substances, etc.

Method used

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  • Conductive multifunctional nanoprobe and preparation method and use thereof
  • Conductive multifunctional nanoprobe and preparation method and use thereof
  • Conductive multifunctional nanoprobe and preparation method and use thereof

Examples

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Embodiment 1

[0043] 1) Preparation of ZIF-8 nanoparticle probes co-modified with xanthine oxidase (XOD), 12-phosphotungstic acid, thionine, gold particles and CYFRA21-1 antibody

[0044] 0.2 mg of xanthine oxidase was added to 1 mL of a 40 mM zinc acetate aqueous solution, followed by stirring for 15 minutes. Then 1 mL of 0.16 mM aqueous 2-methylimidazole was added and stirred for 12 h. The nanoparticle XOD@ZIF-8 was obtained by centrifugation. 1mL 2.5mg mL -1 12-phosphotungstate sodium aqueous solution with 2mL of 5mg mL -1 The XOD@ZIF-8 dispersion was mixed with 12-phosphotungstic acid modified XOD@ZIF-8, sonicated for 1.5 h, stirred for 12 h, and washed by centrifugation. The resulting product was added to 1 mg mL -1 In the thionine aqueous solution, stirred for 12 h, and centrifuged to obtain the thionine-encapsulated nanoprobes. The obtained product was dispersed in 2 mL of secondary water, and 10 μL of 4% NaAuCl was added with stirring 4 Aqueous solution, stirred for 3 h, then 40...

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Abstract

The invention discloses a conductive multifunctional nano-probe and a preparation method thereof. The nano-probe comprises a biological enzyme, a MOFs material coating the biological enzyme, and a heteropolyacid cluster filled in the pores of the MOFs material. (POM) and signaling molecules, gold particles grown in situ on the surface of the MOFs material, and biological antibodies attached to the gold particles. According to the conductive multifunctional nanoprobe of the present invention, the MOFs material filled with POM has good hydrophilicity, which is beneficial to the ion transport in the aqueous solution system, so it has good conductivity. At the same time, the improvement of hydrophilicity is also conducive to the transport of catalytic substrates in the pores, which improves the catalytic activity; POM itself has hydrogen peroxide catalytic activity, and can perform cascade catalytic reactions with biological enzymes to amplify the electrochemical signal of signal substances. ; After the pores of the MOFs material are filled with POM, the remaining space can limit the signal substance molecules, so the signal loading of the probe material is increased.

Description

technical field [0001] The invention belongs to the field of detection materials, in particular to a conductive multifunctional nanoprobe based on a metal-organic framework material, a preparation method thereof, and the use of the probe for electrochemically detecting tumor markers. Background technique [0002] Tumor markers are a class of proteins produced by tumor cells. Different types of tumor cells produce different tumor markers, and the concentrations of tumor markers in the blood of cancer patients at different stages are also different. Therefore, the detection of tumor markers is of great significance for the diagnosis, treatment and prognosis of cancer. At present, the common analytical methods include enzyme-linked immunoassay, fluorescence detection, electrochemical immunoassay, etc. Electrochemical detection has attracted attention due to its advantages of rapid detection, small sample volume, and high sensitivity. However, the current common electrochemica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/58G01N27/327G01N27/48
CPCG01N33/57484G01N33/581G01N33/587G01N27/3278G01N27/48
Inventor 马占芳许阳
Owner CAPITAL NORMAL UNIVERSITY