Method for building animal model for bone metastasis of lung cancer

Abstract

The invention relates to the field of animal models, in particular to a method for building an animal model for bone metastasis of lung cancer through intracardiac injection under ultrasonic guidance.A traditional method for conducting intracardiac injection operation through blind injection is optimized, through ultrasonic guidance, ventriculus sinister is positioned, and lung cancer cell injection is conducted after it is determined that a needle enters the ventriculus sinister. By means of the method, a success rate of building of mouse models for bone metastasis of lung cancer is effectively increased, blindness of intracardiac injection is avoided, and acute bleeding of animals is prevented; and the reliable animal model for bone metastasis of lung cancer is built, so that a novel experimental tool is provided for clinical diagnosis and treatment and basic research of bone metastasis of lung cancer.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for establishing an animal model of lung cancer bone metastasis by intracardiac injection under the guidance of ultrasound. Background technique [0002] Lung cancer is one of the leading causes of patient death worldwide. Although the early diagnosis and clinical treatment of lung cancer have made great progress compared with the past, it cannot be ignored that most lung cancer patients are diagnosed in the middle and late stages, the 5-year survival rate is less than 15%, and more than 90% of lung cancer patients die of metastasis. Bone metastasis is one of the main causes of death in lung cancer patients. Due to the destruction of bone and periosteum, local pain, bone and joint movement disorders, pathological fractures, hypercalcemia, and nerve compression are often caused, resulting in significant quality of life for patients. decline. At present, the occurr...

AI Technical Summary

Problems solved by technology

Although the early diagnosis and clinical treatment of lung cancer have made great progress compared with the past, it cannot be ignored that most lung cancer patients are diagnosed in the middle and late stages, the 5-year survival rate is less than 15%, and more than 90% of lung cancer patients die of metastasis

Creating a reliable animal model is an important basis for the study of tumor mechanisms, but there are few reports on animal models of bone metastasis of lung cancer at home and abroad

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