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Loading promoting device and loading devices

A loading device and sequencing chip technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problem of large consumption of nucleic acid molecules, achieve the effect of increasing the combination ratio and reducing the amount of use

Pending Publication Date: 2020-09-29
MGI TECH CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In addition, the existing technology consumes a large amount of nucleic acid molecules

Method used

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  • Loading promoting device and loading devices
  • Loading promoting device and loading devices
  • Loading promoting device and loading devices

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0062] Example 3: Loading device 3 is used to detect the loading efficiency with the input amount of nucleic acid molecules as 10 fmol, so as to compare with the prior art.

[0063] In this experiment, the input amount of nucleic acid molecules was reduced to one-eighth of the amount used in the prior art, namely 10 fmol. The influence of loading device 3 using mixed electric field loading on the loading efficiency of low-input nucleic acid molecule samples was compared.

[0064] The library used in this experiment is E160 (ie Escherichia coli standard library, the main peak of the insert fragment is 160bp).

[0065] There are 4 groups of experiments in this experiment, which are: control test 1: 80 fmol nucleic acid molecule input, the nucleic acid molecule sample was loaded and incubated for 60 minutes with the existing technology; control test 2: 10 fmol nucleic acid molecule input, the nucleic acid molecule sample was loaded with the existing technology Molecular samples ...

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Abstract

The invention provides a loading promoting device. The loading promoting device comprises an electric field provider, wherein the electric field provider is used for accommodating one or more sequencing chips and exerting an electric field onto the sequencing chips, so that nucleic acid molecules loaded to the sequencing chips are loaded to specific modifying regions of the sequencing chips in anaccelerating manner. The invention also provides loading devices. Through the adoption of the loading promoting device and the loading devices disclosed by the invention, the loading time can be notably shortened, besides, the rate of combining the nucleic acid molecules with the specific modifying regions of the sequencing chips is increased, and the usage quantity of nucleic acid molecule samples is reduced.

Description

technical field [0001] The invention relates to biological sample loading, in particular to a loading promoting device and a loading device. Background technique [0002] The existing nucleic acid molecule loading technology mainly uses the Brownian motion of small molecules to randomly collide, so that the DNA molecules fall onto the specifically modified area on the surface of the sequencing chip. The efficiency of this loading technology for loading DNA samples is relatively low, and it takes a relatively long time The DNA sample can only be loaded onto the specific modification area on the surface of the sequencing chip. At present, the incubation time required to load nucleic acid molecules using this existing loading technology (after the completion of the liquid flow, the required residence time of the reagent) is 60 minutes, plus the fluid flow rate time The hybridization time with the primers takes 90 minutes in the whole process. [0003] Secondly, the existing nu...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6869C12Q2565/518
Inventor 陈莹徐讯章文蔚杨晋王鹏胡书环徐崇钧陈奥王卉
Owner MGI TECH CO LTD
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