Imaging method and device for self-luminous object on biological sample film
A biological sample and imaging device technology, applied in the field of sample analysis, can solve the problems of complex operation process, high cost, and time-consuming imaging, and achieve the effects of simple operation process, low manufacturing cost, and short imaging time-consuming
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Embodiment 1
[0078] Such as figure 1 and figure 2 As shown, the imaging device for a self-luminous object on a biological sample film in this embodiment includes a casing 1 , a photoelectric conversion element 2 and an image correction device 3 .
[0079] The interior of the housing 1 constitutes a darkroom space, wherein the housing 1 includes a light-shielding cover 4 and a base 5, such as image 3 As shown, one side of the shade cover 4 is hinged to one side of the base 5, and the shade cover 4 is in an open state at this moment.
[0080] Such as Figure 4 As shown, when the light-shielding cover 4 and the base 5 are closed, the interior of the housing 1 forms a darkroom space to ensure the biological sample film (in figure 2 The weak signal corresponding to the self-emitting object on the surface is represented by A) to effectively collect.
[0081] Wherein, biological sample membranes include protein membranes, agarose gel blocks, agarose gel strips, polyacrylamide gel gel block...
Embodiment 2
[0103] Such as Figure 7 As shown, the imaging device of the self-luminous object on the biological sample film of this embodiment is a further improvement to Embodiment 1, specifically:
[0104] The imaging device further includes a light source device 6 , and the light source device 6 is arranged in the casing 1 .
[0105] The light source device 6 is arranged at the top position inside the light shielding cover 4;
[0106] The corresponding illumination duration after the light source device 6 is turned on is 10ms-30s, and the illumination duration can also be adjusted according to actual needs.
[0107] The light source device 6 includes a number of LED lamp beads arranged in a dot matrix, a number of lights guided by optical fibers, a number of parallel arranged light tubes, a number of plate-shaped lights, and the like.
[0108] The imaging device also includes a diffuser plate 7 , which is fixed inside the light-shielding cover and directly below the light source devi...
Embodiment 3
[0136] The imaging device of the self-luminous object on the biological sample film of this embodiment is a further improvement on Embodiment 1, specifically:
[0137] This embodiment includes the light source device 6 in Embodiment 2.
[0138] The photoelectric conversion element 2 is also used to obtain bright field images corresponding to the set acquisition time period when the biological sample film is not placed in the housing and a uniform light field is provided by an external light source, that is, the first dark field image, the second dark field image, and the second dark field image. The data acquisition time corresponding to the image and the bright field image are the same;
[0139] The image correction device 3 is also used to correct the second dark field image according to the first dark field image and the bright field image, and obtain the target image corresponding to the self-luminous object, such as Figure 10 As shown, the difference between this pictur...
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Abstract
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