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Staphylococcus sciuri F-E8-1 capable of withstanding high salt environment, application and application method of Staphylococcus sciuri F-E8-1

A technology of Staphylococcus squirrel and an application method, which is applied in the field of microbial treatment of production wastewater, and achieves the effects of high efficiency, short time-consuming and strong industrial adaptability

Active Publication Date: 2020-10-13
SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above process, using three kinds of microorganisms can effectively use wastewater to prepare microbial protein and purify wastewater, but whether these three microorganisms have high salt tolerance and whether they can also purify wastewater well in a high-salt environment capabilities, the above patents do not involve

Method used

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  • Staphylococcus sciuri F-E8-1 capable of withstanding high salt environment, application and application method of Staphylococcus sciuri F-E8-1

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Embodiment 1

[0032] When the present invention handles high-salt fermented wastewater, the bacterial strain adopted is Staphylococcus squirrel (S. Staphylococcus sciuri ) F-E8-1, the strain was deposited in the China Center for Typical Microorganisms Collection on March 6, 2020, with the preservation number CCTCC NO: M 2020052, and the preservation address: No. 202, Wuhan University Collection Center, Wuhan City, Hubei Province, China.

[0033] The aforementioned Staphylococcus squirrel ( Staphylococcus sciuri ) The nucleotide sequence of the 16sRNA of the F-E8-1 strain is shown in Sequence Table 1.

[0034] Add high-salt-resistant and highly efficient Staphylococcus squirrel into the high-salt fermentation wastewater treatment system to achieve the purpose of rapidly degrading the residual sugar in the fermentation wastewater, and after removing the bacteria through solid-liquid separation, concentrate and recover the coarse salt contained in the fermentation wastewater , the specific st...

Embodiment 2

[0038] Staphylococcus squirrel ( Staphylococcus sciuri ) F-E8-1 The steps for treating high-salt fermentation wastewater are as follows:

[0039] Add high-salt-resistant and highly efficient Staphylococcus squirrel to the high-salt fermentation wastewater treatment system to achieve the purpose of rapidly degrading the residual sugar in the fermentation wastewater, and after the bacteria are removed by solid-liquid separation, the coarse salt contained in the fermentation wastewater is concentrated and recovered.

[0040] Take glutamic acid waste water with 21% salt and 1.75% sugar content into the fermentation tank, add peptone to adjust the amino nitrogen content in the waste water to 1.0%, pH: 5.0, fermentation temperature is 33°C, and 1% is added to 12× 10 8 cfu g - 1 Staphylococcus squirrel, after ventilated culture for 35 hours, its sugar content decreased to 0.14%.

[0041] The plate and frame filter press is used for solid-liquid separation, and the plate and frame ...

Embodiment 3

[0043] Staphylococcus squirrel ( Staphylococcus sciuri ) F-E8-1 The steps for treating high-salt fermentation wastewater are as follows:

[0044] Take glutamic acid wastewater containing 12% salt and 1% sugar content and put it into a fermentation tank, add peptone to adjust the amino nitrogen content in the wastewater to 0.55%, pH: 5.8, fermentation temperature is 30°C, and 1% is added to 17× 10 8 cfu g - 1 Staphylococcus squirrel, after 37 hours of ventilated culture, its sugar content dropped to 0.06%.

[0045] Through the solid-liquid separation of the disc centrifuge, the centrifugal force of the centrifuge is 6000g, and the filtrate is firstly evaporated by multiple effects, and then dried in a fluidized bed at a drying temperature of 100°C to obtain crude salt with an ammonium sulfate content of 92.3%.

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Abstract

The invention belongs to the technical field of microbial treatment of production wastewater, and particularly relates to Staphylococcus sciuri capable of withstanding high salt environment, and application of the Staphylococcus sciuri in the treatment of fermentation production wastewater. The Staphylococcus sciuri F-E8-1 capable of withstanding high salt environment, the application of the Staphylococcus sciuri F-E8-1 in the rapid degradation of residual sugar in organic acid and / or amino acid fermentation production wastewater in high salt environment, and an application method are the keyprotection scope. The specific fermentation production wastewater is treated through microbial strains, and the significant advantages such as low operating cost, low consumption, high efficiency, high industrial adaptability, relatively stable operation, convenient operation and management are achieved; the Staphylococcus sciuri has excellent high-salt resistance, and has a good treatment effectduring the treatment of wastewater with high salt content; and the strain of the Staphylococcus sciuri is used to treat high-salt wastewater, the time is consumed less, and the treatment effect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of production wastewater, and specifically relates to a Staphylococcus squirrel squirrel that can tolerate a high-salt environment, and also relates to the application and application method of the above-mentioned Staphylococcus squirrel in the treatment of fermentation production wastewater. Background technique [0002] Fermentation waste liquid refers to the waste liquid discharged after the amino acid is extracted from the fermentation liquid by sedimentation and membrane filtration in the process of amino acid production. It belongs to high-concentration organic waste liquid, which is rich in protein, amino acid, vitamin, sugar and various trace elements. , has the characteristics of high concentration, high suspended solids, high viscosity, and refractory degradation. It is a kind of industrial wastewater that is very difficult to treat. [0003] The glutamic acid industry mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/44C02F103/36C02F101/34
CPCC02F3/34C02F2101/34C02F2103/36C12N1/205C12R2001/44
Inventor 赵晨李宝君李昆仑赵林岳秋林苏乐
Owner SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD
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