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Cross breeding method of hibiscus syriacus and hollyhock

A technology of hybrid breeding and hollyhock, which is applied in the field of hybrid breeding of hibiscus and hollyhock, can solve the problems that the quality cannot be improved, and intergeneric hybridization technology has not been studied.

Active Publication Date: 2020-10-23
河北润丰林业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The propagation methods of hibiscus in the prior art include sowing, layering, cutting, and division, but cutting propagation and division propagation are mainly used in production. Although these methods can propagate hibiscus, they cannot improve its quality.
Even in hybrid breeding, different species of the same genus are used for hybridization, and the technology of intergeneric hybridization has not been studied.

Method used

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  • Cross breeding method of hibiscus syriacus and hollyhock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) take the white flower single-petal hibiscus as the female parent, remove the stamens before flowering, and bag the flowers with a white sulfuric acid paper bag of 15cm * 8cm after the stamens are removed;

[0055] (2) Dry the stamens in step (1) to a water content of 10%, collect pollen, spray 70% alcohol on the pollen until the pollen is moist, dry it again in the same way after the alcohol evaporates, and put it in a -78°C Frozen in ultra-low temperature refrigerator, used as induced pollen, for later use;

[0056] (3) using the yellow-flowered hollyhock as the male parent, collecting pollen after flowering, mixing the collected male parent pollen with the induced pollen at a volume ratio of 0.9:1.1, as mixed pollen, for subsequent use;

[0057] (4) Mix the leaves of elm elm and the PBS buffer solution with a pH value of 7.1 according to the dosage ratio of 1g: 8mL, grind, centrifuge at 10000r / min for 6min, and use the yellow viscous substance between the supernat...

Embodiment 2

[0061] (1) take the white flower single-petal hibiscus as the female parent, remove the stamens before flowering, and bag the flowers with a white sulfuric acid paper bag of 18cm * 10cm after the stamens are removed;

[0062] (2) Dry the stamens in step (1) to a water content of 12.5%, collect pollen, spray 75% alcohol on the pollen until the pollen is moist, dry the stamens again in the same way after the alcohol volatilizes, and put them in a -79°C Frozen in ultra-low temperature refrigerator, used as induced pollen, for later use;

[0063] (3) using hollyhock chrysanthemum as the male parent, collecting pollen after flowering, mixing the collected male parent pollen with the induced pollen at a volume ratio of 1:1, as mixed pollen, for subsequent use;

[0064] (4) Mix the leaves of Elm elm and the PBS buffer solution with a pH value of 7.2 according to the dosage ratio of 1g: 10mL, grind, centrifuge at 12000r / min for 5min, and use the yellow viscous substance between the su...

Embodiment 3

[0068] (1) take the white flower single-petal hibiscus as the female parent, remove the stamens before flowering, and bag the flowers with a white sulfuric acid paper bag of 20cm * 12cm after removing the stamens;

[0069] (2) Dry the stamens in step (1) to a water content of 15%, collect the pollen, spray 99.5% alcohol on the pollen until the pollen is wet, dry it again in the same way after the alcohol volatilizes, and put it in a -80°C Frozen in ultra-low temperature refrigerator, used as induced pollen, for later use;

[0070] (3) using the yellow-flowered hollyhock as the male parent, collect pollen after flowering, mix the collected male parent pollen with the induced pollen at a volume ratio of 1.1:0.9, and use it as mixed pollen for subsequent use;

[0071] (4) Mix the leaves of Elm elm and the PBS buffer solution with a pH value of 7.3 according to the dosage ratio of 1g: 12mL, grind, centrifuge at 14000r / min for 4min, and use the yellow viscous substance between the ...

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Abstract

The invention relates to the technical field of plant crossbreeding, and provides a cross breeding method of hibiscus syriacus and hollyhock. The method comprises the following steps: (1) taking hibiscus syriacus as a female parent, and removing stamens before flowering; (2) drying the stamens in the step (1), collecting pollen, inactivating the pollen to serve as induced pollen for later use; (3)taking hollyhock as a male parent, collecting pollen after blooming, and mixing the collected male parent pollen with the induced pollen to obtain mixed pollen for later use; (4) mixing white elm leaves with a buffer solution, performing grinding and centrifuging, and taking the middle layer as an adhesive for later use; (5) taking stigmas of male parent plants, and grinding the stigmas into pulpas a stigma recognition protein for later use; and (6) sequentially smearing the female parent stigma with the adhesive, the stigma recognition protein and the mixed pollen to finish pollination. Thecross breeding method of the invention can effectively carry out intergeneric hybridization of hibiscus syriacus and hollyhock, a malvaceae new variety is created, and the maturing rate of intergeneric hybridization is increased.

Description

technical field [0001] The invention relates to the technical field of plant hybrid breeding, in particular to a method for hybrid breeding of hibiscus and hollyhock. Background technique [0002] Hibiscus is a deciduous shrub of Malvaceae Malvaceae, with a height of 3 to 4 meters. There are more than 200 species of Hibiscus plants, which are distributed in tropical and subtropical regions. There are 24 species and 16 varieties or variants in our country, which are produced all over the country. In my country, hibiscus plants are widely used in gardens. They are a kind of shrub species that are very common in gardens. They are native to the provinces in central China and are cultivated in various places. It can be used as a flower hedge in the garden, and can be planted alone or in clusters. The main reason is that there are fewer flowering plants in summer and autumn, while hibiscus plants can bloom from early July to the end of September, and the flowers are gorgeous. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 刘易超陈丽英刘冬云李银华樊彦聪代嵩华黄印朋付晓燕霍对对郝明亮
Owner 河北润丰林业科技有限公司
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