Detection method for correlation between GARNL1 gene and chicken breed testis traits and application

A detection method and correlation technology, applied in the field of genetic engineering

Active Publication Date: 2020-10-23
NANCHANG NORMAL UNIV
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Problems solved by technology

Shen et al. (2012) found that the rs14532831 locus was significantly correlated with the age at first laying of ch

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  • Detection method for correlation between GARNL1 gene and chicken breed testis traits and application
  • Detection method for correlation between GARNL1 gene and chicken breed testis traits and application

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Embodiment Construction

[0020] Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation to the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and embodiments of the present invention.

[0021] It should be understood that the terms described in the present invention are only used to describe specific embodiments and are not used to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intermediate value within the stated range and any other stated value or intermediate value within the stated range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included o...

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Abstract

The invention discloses a detection method for correlation between GARNL1 gene and chicken breed testis traits and application, and belongs to the field of genetic engineering. The method comprises the following steps: firstly, extracting genome DNA of a to-be-detected chicken breed, amplifying upstream and downstream 463bp fragments of an rs14532831 site of a 5' regulation region of the GARNL1 gene, performing RFLP typing on an obtained PCR product, and performing statistical analysis on correlation between different genotypes of the rs16492031 site of the GARNL1 gene and cock testis traits.According to the invention, the correlation relationship between the polymorphism of the rs14532831 site of the chicken breed GARNL1 gene and the testis traits of the chicken breed is detected, and the relationship and the method are used as the means for breeding and marking chicken breeds. Compared with existing slaughter breeding, the breeding method is low in cost, easy and convenient to operate and accurate in result.

Description

Technical field [0001] The invention relates to the field of genetic engineering, in particular to a detection method and application of the correlation between the GARNL1 gene and the testis traits of chicken breeds. Background technique [0002] The GTPase-activating Rap / RanGAP domain-like 1 protein (GARNL1) gene is currently rarely studied in chickens (Chen et al., 2006, 2007; Shen et al., 2012), and focuses on the relationship between the gene and hen reproductive performance such as egg production , In terms of nesting, age at the start of labor, etc. Studies of human GARNL1 have shown that it is associated with neurophenotype and family basal ganglia calcification (Familial idiopathic basal ganglia calcification, IBGC; also known as Fahr disease) (Schwarzbraun et al., 2004), and is associated with brain developmental arrest (Shimojima et al., 2009). So far, the reproductive performance of GARNL1 gene and native chicken breeders has not been reported. [0003] The testis is...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 周敏朱学农谭玉文贡继尚许继国文正亚
Owner NANCHANG NORMAL UNIV
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