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Biocontrol microbial agent for preventing and treating stem rot and gummosis and preparation method and application thereof

A biocontrol agent and stem rot technology, which is applied in the directions of microorganism-based methods, spore use methods, botanical equipment and methods, etc. problems, to inhibit growth and reproduction, enhance antioxidant capacity, and promote resistance to stem rot and gum disease

Inactive Publication Date: 2020-10-30
SUZHOU RES INST SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this bacterial strain is not ideal for the prevention and treatment of stem rot, and has great limitations in use, and cannot be widely used in crops.

Method used

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  • Biocontrol microbial agent for preventing and treating stem rot and gummosis and preparation method and application thereof
  • Biocontrol microbial agent for preventing and treating stem rot and gummosis and preparation method and application thereof
  • Biocontrol microbial agent for preventing and treating stem rot and gummosis and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A mixed fermentation liquid of Bacillus subtilis and Trichoderma longbranchs, the preparation steps are as follows:

[0065](1) Preparation of Bacillus subtilis spore suspension: use an inoculation loop to streak and inoculate the refrigerated Bacillus subtilis strains on the flat plate LB medium in an ultra-clean workbench, culture at 37°C for 24 hours, and then inoculate them in an ultra-clean workbench. Use an inoculation loop to pick a colony with a diameter of about 0.5 cm on the flat LB medium in the clean workbench, inoculate it in the liquid LB medium at a ratio of 1 / 100 mL, and culture it at 180 rpm and 37 °C for 24 hours, and the concentration of viable spores is 8×10 8 Individual / mL, make Bacillus subtilis spore suspension;

[0066] (2) Preparation of Trichoderma longibrachiata spore suspension: use an inoculation loop to inoculate the refrigerated Trichoderma longibrachiatum strains on the flat PDA medium in a super-clean workbench, culture at 28°C for 48 ho...

Embodiment 2

[0069] A biocontrol bacterial agent for preventing and treating crop stalk rot and gummosis, the preparation steps are as follows:

[0070] (1) Preparation of Bacillus subtilis spore suspension: use an inoculation loop to streak and inoculate the refrigerated Bacillus subtilis strains on the flat plate LB medium in an ultra-clean workbench, culture at 37°C for 24 hours, and then inoculate them in an ultra-clean workbench. Use an inoculation loop to pick a colony with a diameter of about 0.5 cm on the flat LB medium in the clean workbench, inoculate it in the liquid LB medium at a ratio of 1 / 100 mL, and culture it at 180 rpm and 37 °C for 24 hours, and the concentration of viable spores is 8×10 8 Individual / mL, make Bacillus subtilis spore suspension;

[0071] (2) Preparation of Trichoderma longibrachiata spore suspension: use an inoculation loop to inoculate the refrigerated Trichoderma longibrachiatum strains on the flat PDA medium in a super-clean workbench, culture at 28°C ...

Embodiment 3

[0076] A biocontrol bacterial agent for preventing and treating crop stalk rot and gummosis, the preparation steps are as follows:

[0077] (1) Preparation of Bacillus subtilis spore suspension: use an inoculation loop to streak and inoculate the refrigerated Bacillus subtilis strains on the flat plate LB medium in an ultra-clean workbench, culture at 37°C for 24 hours, and then inoculate them in an ultra-clean workbench. Use an inoculation loop to pick activated colonies with a diameter of about 0.5 cm on the flat LB medium in the clean workbench, inoculate them in the liquid LB medium at a ratio of 1 colony / 100 mL, culture at 180 rpm, 37 ° C for 24 hours, and the concentration of viable spores is 8 ×10 8 Individual / mL, make Bacillus subtilis spore suspension;

[0078] (2) Preparation of Trichoderma longibrachiata spore suspension: use an inoculation loop to inoculate the refrigerated Trichoderma longibrachiatum strains on the flat PDA medium in a super-clean workbench, cult...

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Abstract

The invention discloses a biocontrol microbial agent for preventing and treating stem rot and gummosis. The biocontrol microbial agent contains 99.0-99.5% by weight of mixed fermentation liquor of bacillus subtilis and trichoderma longibrachiatum and 0.5-1.0% by weight of extracellular crude polysaccharide, the invention further provides a preparation method and application of the biocontrol microbial agent for preventing and treating stem rot and gummosis of crops. The biocontrol microbial agent prepared by mixed fermentation of bacillus subtilis and trichoderma longibrachiatum has very obvious prevention and treatment effects on stem rot and gummosis of crops, has the characteristics of being environmentally friendly, high in safety and wide in prevention and treatment spectrum, and caneffectively reduce the use of chemical pesticides and environmental pollution caused by the use of the chemical pesticides.

Description

technical field [0001] The invention relates to a biocontrol bacterial agent for preventing and treating stem rot and gummosis, a preparation method and application thereof, and belongs to the technical field of microbial biological control. Background technique [0002] Stem rot and gummosis of crops are common and serious in our country, threatening the stable and high yield of some vegetables and fruits, and causing huge economic losses to farmers. There are many reasons for stem rot, and the most serious type is fungal stem rot. Fungal stem rot is a type of disease that causes root and stem rot caused by a variety of pathogenic bacteria alone or in combination. , mainly caused by Pythium, Anthracnose, Fusarium infection. The cortex at the base of the stem of the diseased plant is light brown or dark brown. Some plants lose water and shrink, and the leaves turn yellow, wilt, or even die. The current stem rot prevention method is mainly chemical agents, for example, CN10...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N63/38A01N43/16A01P3/00C12N1/20C12N1/14C12N3/00C12P39/00C12P19/04C12R1/125C12R1/885
CPCA01N43/16C12N1/14C12N1/20C12N3/00C12P19/04C12P39/00
Inventor 马群飞丛韫喆鹿艳冯丽婷张鹏英陈靠山
Owner SUZHOU RES INST SHANDONG UNIV
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