Biological agent for reducing piglet diarrhea
A technology for biological agents and piglet diarrhea, applied in the field of animal husbandry biological agents, can solve problems such as increased bacterial resistance, decreased animal immunity, and threats to human health, and achieve the effect of promoting proliferation
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Embodiment 1
[0044] (1) Mix and pulverize saxifrage, Corydalis tuber and duckweed according to the ratio of 3:1:3 to obtain compound plant powder;
[0045] (2) Add deionized water to the crushed composite plant powder according to the ratio of solid to liquid 1:15, soak at 60°C for 3 hours;
[0046] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;
[0047] (3) Add 85% ethanol, alcohol precipitation for 24 hours;
[0048] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain complex polysaccharide A.
Embodiment 2
[0050] (1) Mix and pulverize saxifrage, Corydalis tuber and duckweed according to the ratio of 2:3:3 to obtain compound plant powder;
[0051] (2) Add deionized water to the crushed composite plant powder according to the ratio of solid to liquid 1:15, soak at 60°C for 3 hours;
[0052] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;
[0053] (3) Add 85% ethanol, alcohol precipitation for 24 hours;
[0054] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain complex polysaccharide B.
Embodiment 3
[0056] (1) Mix and pulverize saxifrage, corydalis tuber and duckweed according to the ratio of 2:1:5 to obtain compound plant powder;
[0057] (2) Add deionized water to the crushed composite plant powder according to the ratio of solid to liquid 1:15, soak at 60°C for 3 hours;
[0058] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;
[0059] (3) Add 85% ethanol, alcohol precipitation for 24 hours;
[0060] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain complex polysaccharide C.
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