Biological agent for reducing piglet diarrhea

A technology for biological agents and piglet diarrhea, applied in the field of animal husbandry biological agents, can solve problems such as increased bacterial resistance, decreased animal immunity, and threats to human health, and achieve the effect of promoting proliferation

Active Publication Date: 2020-10-30
QINGDAO PUXING BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of antibiotics will lead to enhanced bacterial resistance, decreased animal immunity, and will affect the normal

Method used

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  • Biological agent for reducing piglet diarrhea
  • Biological agent for reducing piglet diarrhea

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1

[0044] (1) Saxifraga, Corydalis tuber and duckweed are mixed and crushed according to 3:1:3 to obtain compound plant powder;

[0045] (2) Add deionized water to the crushed composite plant powder at a material-to-liquid ratio of 1:15, soak for 3 hours at 60°C;

[0046] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;

[0047] (3) Add 85% ethanol, and alcohol precipitation for 24h;

[0048] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain compound polysaccharide A.

Example Embodiment

[0049] Example 2

[0050] (1) Saxifraga, Corydalis tuber and duckweed are mixed and crushed according to 2:3:3 to obtain compound plant powder;

[0051] (2) Add deionized water to the crushed composite plant powder at a material-to-liquid ratio of 1:15, soak for 3 hours at 60°C;

[0052] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;

[0053] (3) Add 85% ethanol, and alcohol precipitation for 24h;

[0054] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain compound polysaccharide B.

Example Embodiment

[0055] Example 3

[0056] (1) Saxifraga, Corydalis tuber and duckweed are mixed and crushed according to 2:1:5 to obtain composite plant powder;

[0057] (2) Add deionized water to the crushed composite plant powder at a material-to-liquid ratio of 1:15, soak for 3 hours at 60°C;

[0058] (2) Centrifuge, collect the supernatant, and distill under reduced pressure to 10% of the original volume;

[0059] (3) Add 85% ethanol, and alcohol precipitation for 24h;

[0060] (4) Add Sevage reagent, centrifuge to remove protein, repeat 5 times to obtain compound polysaccharide C.

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Abstract

The invention aims to provide a biological agent for reducing piglet diarrhea, and belongs to the technical field of livestock biological agents. The biological agent provided by the invention is compound polysaccharide consisting of saxifraga stolonifera polysaccharide, corydalis tuber polysaccharide and duckweed polysaccharide. The composite preparation provided by the invention has a relativelygood inhibition effect on escherichia coli, staphylococcus aureus and salmonella; secondly, the biological agent provided by the invention can effectively promote proliferation of porcine small intestine epithelial cells and expression of porcine small intestine epithelial cell connexin; in addition, the biological agent provided by the invention can effectively reduce diarrhea of weaned piglets.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry biological preparations, in particular to a biological preparation for reducing diarrhea in piglets. Background technique [0002] In modern intensive pig farming environment, early weaning of piglets is widely used in order to maximize the reproductive performance of sows and reduce the possibility of vertical transmission of diseases to piglets. However, because piglets need to obtain a large amount of antibodies and easily digestible lactose and milk protein from breast milk, and the digestive system of piglets is not yet mature, the regulation mechanism of the endocrine system has not been fully established, and the immune function is low. Therefore, due to changes in feeding conditions during the weaning process of piglets, a series of physiological changes will occur, such as digestive disorders, diarrhea, slow growth and so on. Weaning stress can lead to changes in intestinal morp...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K50/60A23K20/163A23K10/30A61K31/715A61P1/12A61P31/04A61P1/00C08B37/00
CPCA23K50/30A23K50/60A23K20/163A23K10/30A61K31/715A61P1/12A61P31/04A61P1/00C08B37/0003A61K2300/00Y02A50/30Y02P60/87
Inventor 吕广宙王巧莉李久峰齐国锋李红玲韩娟
Owner QINGDAO PUXING BIO TECH CO LTD
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