An electrochemical analysis method for simultaneous detection of glucose and insulin
An electrochemical analysis and insulin technology, applied in the field of electrochemical detection and electrode modification, can solve the problem of inability to measure the concentration information, and achieve the effect of on-site instant detection, simple modification method, and convenient operation
Active Publication Date: 2021-08-31
YANSHAN UNIV
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Problems solved by technology
The existing electrochemical detection technology of glucose or insulin cannot use the same electrode to measure the concentration information of the two in the mixed solution, but it is necessary to design a dual-channel electrode system, each channel can only detect one substance
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Embodiment Construction
[0037] The invention provides an electrochemical detection method that can be used for the simultaneous determination of glucose and insulin, as shown in the attached figure 1 As shown, it mainly includes:
[0038] In step S101, a three-electrode system is selected as a sensor for collecting electrochemical data, namely a working electrode, an auxiliary electrode, and a reference electrode, wherein both the working electrode and the auxiliary electrode are carbon electrodes, and the reference electrode is a silver electrode. Carbon electrodes have the advantages of wide potential range and good sensitivity. The overall length of the screen-printed three-electrode system is 30 mm, the thickness is 0.36 mm, and the width is 0.6 mm. The working electrode is circular with a radius of 2.5 mm. The selected screen-printed electrodes are small in size, low in cost, multifunctional, and easy to achieve functional modification of the electrodes.
[0039] Step S102, according to the re...
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Abstract
The invention discloses an electrochemical analysis method for simultaneous detection of glucose and insulin. The three-electrode system is used to collect electrochemical data, and the working electrode is modified with nickel hydroxide by an electrodeposition method. Insulin and glucose solutions were detected by cyclic voltammetry in electrochemistry, which proved that the modified electrode could detect the concentrations of insulin and glucose. The detection limit and sensitivity of insulin and glucose were determined by chronoamperometry. Prepare a mixed solution of insulin and glucose, use cyclic voltammetry to perform electrochemical detection of the mixed solution, extract the physical significance feature points on the cyclic voltammetry curve, use the regression method of machine learning to decouple, and obtain the mixed solution of insulin and glucose respectively. concentration in solution. Compared with the prior art, the device of the present invention is simple, low in cost, convenient in operation and good in portability, and is beneficial to realizing family prevention and preliminary clinical diagnosis of diabetes.
Description
technical field [0001] The invention relates to the fields of electrochemical detection and electrode modification, in particular to an electrochemical analysis method capable of simultaneously detecting two different substances of glucose and insulin. Background technique [0002] Glucose in the blood provides energy for various activities of the body. It can be used normally by the body only when it maintains a dynamic balance within a certain range. Insulin is the only hormone that can lower blood sugar in the body. Insufficient insulin secretion or dysfunction in the body can lead to Excessive blood sugar levels can lead to diabetes and a series of complications. Oral glucose tolerance test (OGTT) and fasting blood glucose (FPG) are commonly used clinically for the diagnosis of diabetes. However, blood glucose testing is not the basis for early diagnosis of diabetes, nor can it accurately determine the changes in the secretory function of pancreatic β cells. If the insu...
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IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N27/416
CPCG01N27/308G01N27/3277G01N27/3278G01N27/416G01N27/48
Inventor 詹志坤张宏宇
Owner YANSHAN UNIV