Stainless steel self-sealing gel purification column device

Abstract

The invention provides a stainless steel self-sealing gel purification column device, which comprises a stainless steel column; a gel filler can be contained in the stainless steel column; the liquidinlet end of the stainless steel column tube is fixed through a liquid inlet end sealing nut, and the liquid outlet end of the stainless steel column tube is fixed through a liquid outlet end sealingnut; a liquid inlet end one-way control mechanism is arranged at the liquid inlet end of the stainless steel column tube, so that liquid can flow into the stainless steel column tube from the liquid inlet end of the stainless steel column tube and cannot flow out reversely; and a liquid outlet end one-way control mechanism is arranged at the liquid outlet end of the stainless steel column tube, sothat liquid in the stainless steel column tube can only flow out of the liquid outlet end of the stainless steel column tube and cannot flow into the stainless steel column tube reversely. Accordingto the principle of a one-way valve, when a mobile phase with a certain flow rate passes through the gel column, the mobile phase can normally pass through the gel column; and when no mobile phase passes through the gel column, the mobile phase is sealed by using the one-way stop valve, so that the loss and volatilization of the mobile phase in the gel column are reduced.

Description

technical field [0001] The invention relates to a stainless steel self-sealing gel purification column device, which is mainly used for the gel purification technology of food and drug environment safety, especially the purification method of food environment detection. By using the invention, misoperation by users, loss of solvent and decrease of column efficiency can be avoided. Background technique [0002] The stainless steel self-sealing gel column has a high separation effect on macromolecular substances through the principle of size exclusion, and is also widely used to separate small molecular compounds. In terms of food environmental safety testing, gel purification is widely used to remove macromolecules such as oil, protein and pigment. [0003] The commonly used gel column is packed with glass, which has poor compression resistance and loose packing, which wastes solvent. Once the system pressure is too high, there is a risk of glass breakage. The column can r...

AI Technical Summary

Problems solved by technology

If the direction of the column is not installed correctly when it is used again, the fluidity will backwash the gel column, which will affect the service life of the gel column

Because the packing of the gel column is relatively expensive, if the packing collapses and the column efficiency decreases, the packing must be replaced, resulting in economic losses

[0005] There will also be stainless steel columns in the market, but in addition to solving the problem of pressure resistance, other problems such as the inability to flow liquid in one direction, no self-sealing function, the mobile phase is prone to reflux and leakage still exist, which will affect the service life of the gel column

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