Breeding method for clematis seedling tissue culture

Abstract

The invention relates to the field of clematis seedling propagation, and particularly discloses a propagation method for clematis seedling tissue culture. The breeding method for clematis seedling tissue culture comprises the following steps that S1, explants are selected, specifically, a single stem segment with axillary buds is cut to serve as the explants, the single stem segment with axillarybuds contains 2-3 axillary buds, and the length of the stem segment is 1.5-2 cm; S2, explant disinfection is conducted, specifically, the explant is wiped with medical alcohol, then the explant is immersed into a mixed solution of bleaching water and sterile water in a ratio of 1:5 for 20-30 minutes, and washing is conducted with sterile water for 3-4 times each for 2-5 min; S3, culture is started; S4, multiplication culture is conducted; and S5, rooting culture is conducted. According to the breeding method, plant sprouts can be effectively induced, cluster buds and roots are generated, the possibility that the plants generate calluses and variation is reduced, and the survival rate of seedlings is ensured to a certain extent.

Description

technical field [0001] The application relates to the field of clematis seedling breeding, more specifically, it relates to a breeding method of clematis seedling tissue culture. Background technique [0002] Clematis is a plant of the Ranunculaceae family. It is a well-known high-end ornamental flower in the world. It has high ornamental value and has pharmacological effects such as antibacterial, antitumor, anti-inflammatory, analgesic, and antiviral. [0003] At present, the following methods are often used for the breeding of clematis: sowing: sowing in spring, it can germinate and grow in about 3-4 weeks, sowing in autumn, it will germinate when the spring is warm; Rooting within 1 year; Cuttings: Take semi-mature branches from July to August, take internodes for cuttings, put them in 3-inch pots after rooting, overwinter in an antifreeze hotbed or greenhouse, change 4-5 inches in spring, move outside, and plant at the end of October . [0004] For the above-mentioned...

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Problems solved by technology

[0004] For the above-mentioned related technologies, the inventor believes that although the current breeding methods can breed clematis to a certain extent, the inventor finds that: adopting the sowing method to breed clematis requires more seeds. High, the survival rate of seeds is low; the layering method is used to breed clematis, the environmental requirements for branches and layering are high, and the survival rate is also low; the cutting method is used to breed clematis, the rooting of cuttings is not stable enough, and the cutting time is short , The proportion of cutting substrates and the control of cutting microenvironment have a great influence on the rooting rate, which will lead to a low final survival rate

No matter which of the above methods is used to breed clematis, the final survival rate cannot well meet the needs of enterprises to breed clematis in large quantities

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