Magnetic COFs based immobilized enzyme, synthetic method and applications of immobilized enzyme in preparation of biodiesel

A technology of immobilizing enzymes and synthesis methods, applied in the directions of immobilization on or in inorganic carriers, biofuels, hydrolase, etc., can solve the problems of low activity of biocatalysts, poor stability of carriers, etc. Stable, high-yield effects

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Problems solved by technology

[0009] The purpose of the present invention is to provide an immobilized enzyme based on magnetic COFs, a synthesis method and its application in the preparation of biodiesel, which can solve the problems of low biocatalyst activity and poor carrier stability, and also break through the use of COFs materials for enzymes Research on the fixed performance of

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  • Magnetic COFs based immobilized enzyme, synthetic method and applications of immobilized enzyme in preparation of biodiesel
  • Magnetic COFs based immobilized enzyme, synthetic method and applications of immobilized enzyme in preparation of biodiesel
  • Magnetic COFs based immobilized enzyme, synthetic method and applications of immobilized enzyme in preparation of biodiesel

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[0049] The present invention provides a method for synthesizing immobilized enzymes based on magnetic COFs, specifically including the synthesis of magnetic COFs and the immobilization of enzymes, wherein the synthesis of magnetic COFs includes the following steps:

[0050] (1) Weigh and dissolve

[0051] Weigh raw material Fe in proportion 3 o 4 Magnetic nanoparticles, 2,5-dimethoxyterephthalaldehyde and 1,3,5-tris(aminophenyl)benzene, dissolved in a solvent;

[0052] (2) Catalysis

[0053] Sonicate the above solution, add the catalyst dropwise and let it stand overnight to obtain solid particles, wherein the ultrasonic time is 5-10min;

[0054] (3) washing

[0055] Washing the solid particles obtained in step (2) after separation with a magnet;

[0056] (4) dry

[0057] Dry the washed solid particles in vacuum at 50°C for 24 hours to obtain Fe 3 o 4 @COF-OMe.

[0058] Among them, the specific steps of enzyme immobilization are: Fe 3 o 4 @COF-OMe was dispersed in P...

Embodiment 1

[0060] A synthetic method for immobilized enzymes based on magnetic COFs, including the synthesis of core-shell magnetic COFs (Fe 3 o 4 @COF-OMe):

[0061] 1. Weigh 0.24mmol of 2,5-dimethoxyterephthalaldehyde, 0.16mmol of 1,3,5-tris(aminophenyl)benzene and 30mg of magnetic Fe 3 o 4 Nanoparticles, placed in 20mL acetonitrile solution;

[0062] 2. Sonicate the system for 5 minutes to make insoluble particles such as magnetic Fe 3 o 4 The particles are dispersed in the system;

[0063] 3. Slowly add 1.5mL of acetic acid dropwise while ultrasonicating, and let stand overnight after the addition is complete;

[0064] 4. Separate the obtained solid particles from the system with a magnet, wash with anhydrous tetrahydrofuran and methanol for 3 times, and then extract and wash with anhydrous tetrahydrofuran for 48 hours in a Soxhlet extractor;

[0065] 5. Collect solid particles and dry them in vacuum at 50°C for 24 hours to obtain yellow magnetic solid particles (Fe 3 o 4 @C...

Embodiment 2

[0068] A synthetic method for immobilized enzymes based on magnetic COFs, including the synthesis of core-shell magnetic COFs (Fe 3 o 4 @COF-OMe):

[0069] 1. Weigh 0.12mmol of 2,5-dimethoxyterephthalaldehyde, 0.08mmol of 1,3,5-tris(aminophenyl)benzene and 15mg of magnetic Fe 3 o 4 Nanoparticles, placed in 20mL acetonitrile solution;

[0070] 2. Sonicate the system for 8 minutes to make insoluble particles such as magnetic Fe 3 o 4 The particles are dispersed in the system;

[0071] 3. Slowly add 1.5mL of acetic acid dropwise while ultrasonicating, and let stand overnight after the addition is complete;

[0072] 4. Separate the obtained solid particles from the system with a magnet, wash with anhydrous tetrahydrofuran and methanol for 3 times, and then extract and wash with anhydrous tetrahydrofuran for 48 hours in a Soxhlet extractor;

[0073] 5. Collect solid particles and dry them in vacuum at 50°C for 24 hours to obtain yellow magnetic solid particles (Fe 3 o 4 @C...

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Abstract

The invention discloses a magnetic COFs based immobilized enzyme, a synthetic method and applications of the immobilized enzyme in preparation of biodiesel. The immobilized enzyme includes nanoparticles with core-shell structures and immobilized hydrolase; the core is Fe3O4 magnetic nanoparticles, and the shell is COFs; and the immobilized hydrolase is RML. The synthetic method includes the synthesis of the magnetic COFs and the immobilization of the enzymes; a magnetic COFs material is synthesized and then used to immobilize the RML so as to obtain the immobilized enzyme RML@Fe3O4@COF-OMe. The immobilized enzyme is good in stability; the characteristics of the RML of high catalytic activity can be reserved; and the high yield effects of the immobilized enzyme in preparation of the biodiesel can be achieved.

Description

technical field [0001] The invention relates to the technical field of immobilized enzymes, in particular to an immobilized enzyme based on magnetic COFs, a synthesis method and its application in the preparation of biodiesel. Background technique [0002] At present, under the guidance of the principles of green chemistry and sustainable development, the development of new renewable energy has become a research hotspot. Biodiesel is based on good biorenewability and biodegradability, and its development and preparation are of great significance. Nowadays, in the process of preparing biodiesel, commonly used chemical catalysts such as acids and bases have many shortcomings in the catalytic process: 1. The chemical catalysts are relatively polluted; 2. It is difficult to separate from the system and the recovery effect is not good; 3. The conditions for chemical catalysts to catalyze reactions are harsh. [0003] Therefore, the preparation of biodiesel based on biocatalysts...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12N11/14C12P7/64C11C3/10
CPCC12N9/20C12N11/14C12P7/649C11C3/10Y02E50/10
Inventor 王娜余孝其周子文
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