Unlock instant, AI-driven research and patent intelligence for your innovation.

Mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody capable of being applied to tumor cell capture

A monoclonal antibody, transmembrane glycoprotein technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, anti-animal/human immunoglobulin, immunoglobulin, etc., can solve the lack of antibodies tools, no data to suggest issues such as enrichment of live tumor cells to achieve high-affinity effects

Pending Publication Date: 2020-12-18
普众发现医药科技(上海)有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CD142 is involved in placental development in early pregnancy, and is related to the proliferation, differentiation and apoptosis of trophoblast cells. Currently, there are few antibody products on the market that use CD142 as a target carrier, and there is no data showing that they can be used to enrich live tumor cells
At the same time, there is a lack of effective antibody tools for this research field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody capable of being applied to tumor cell capture
  • Mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody capable of being applied to tumor cell capture
  • Mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody capable of being applied to tumor cell capture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051]It should be noted that the embodiments in this application and the features in the embodiments can be combined with each other if there is no conflict. The following will clarify the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. A complete description. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0052]Referencefigure 1 A preferred embodiment of the present invention provides a mouse anti-cellular single-chain transmembrane glycoprotein CD142 monoclonal antibody that can be used to capture tumor cells. The process includes the following steps:

[0053]S1, Protein QC: purchase commercialized protei...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a monoclonal antibody, in particular to a mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody capable of being applied to tumor cell capture. A process of the mouse anti-cell-single-chain-transmembrane-glycoprotein CD142 monoclonal antibody comprises the following steps of: S1, protein QC; S2, animal immunization; S3, serum detection and screening; S4, fusion and screening; S5, subcloning and screening; S6, preparation of antibody supernatant; and S7, antibody verification. The mouse anti-cell-single-chain-transmembrane-glycoprotein CD142monoclonal antibody capable of being applied to tumor cell capture, which is disclosed by the invention, has the characteristics of high affinity, high specificity, multiple application scenes and thelike, and can be applied to living cell enrichment.

Description

Technical field[0001]The present invention relates to a monoclonal antibody, in particular to a mouse anti-cellular single-chain transmembrane glycoprotein CD142 monoclonal antibody that can be applied to capture tumor cells.Background technique[0002]CD142 is a transmembrane single-chain glycoprotein related to a family of proteins called type II cytokine receptors. Members of this receptor family are activated by cytokines. Cytokines are small proteins that can affect the behavior of white blood cells. It was also found that the combination of Vila and TF initiates the intracellular signaling process. The signal transduction function of TF / VIIa plays a role in angiogenesis and apoptosis. Pro-inflammatory and pro-angiogenic responses are activated by TF / VIIa-mediated cleavage of protease-activated receptor 2 (PAR2). [8] EphB2 and EphA2 of the Eph tyrosine kinase receptor (RTK) family can also be cleaved by TF / VIIa. CD142 is involved in placental development in early pregnancy and is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C07K16/06C12N5/20
CPCC07K16/2896C07K16/06C07K2317/56
Inventor 孟逊
Owner 普众发现医药科技(上海)有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com