Method for culturing dedifferentiated or undifferentiated thyroid carcinoma organ, and thyroid carcinoma culture medium
A thyroid cancer and culture medium technology, applied in endocrine organ cells, biochemical equipment and methods, 3D culture, etc., can solve problems such as lack of iodine uptake ability, poor prognosis of thyroid cancer patients, and DTC dedifferentiation
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Embodiment 1
[0048] This example demonstrates the culture of undifferentiated thyroid carcinoma organoids.
[0049] (1) Preservation and transportation of undifferentiated thyroid cancer tissue samples
[0050] The puncture tissues of patients with clinically confirmed anaplastic thyroid carcinoma were obtained as anaplastic thyroid carcinoma tissue samples. Undifferentiated thyroid cancer tissue samples were stored in thyroid cancer medium and transported to the operating room within 48 hours of cold chain at 4 degrees.
[0051] (2) Obtaining undifferentiated thyroid cancer tissue cells
[0052] Place the cleaned undifferentiated thyroid carcinoma tissue sample in a sterile petri dish, add the enzymatic hydrolysis solution (DMEM / F12 containing 2 volume % FBS and 2 mg / ml collagen hydrolytic enzyme) after filtration sterilization and warm bath at 37 °C culture medium), and use sterile instruments to cut the undifferentiated thyroid cancer tissue sample into minced shape to obtain the enzy...
Embodiment 2
[0058] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 5 mM, and the concentration of BM-Cyclin was 5 μg / ml.
Embodiment 3
[0060] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 20 mM, and the concentration of BM-Cyclin was 30 μg / ml.
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