Method for culturing dedifferentiated or undifferentiated thyroid carcinoma organ, and thyroid carcinoma culture medium

A thyroid cancer and culture medium technology, applied in endocrine organ cells, biochemical equipment and methods, 3D culture, etc., can solve problems such as lack of iodine uptake ability, poor prognosis of thyroid cancer patients, and DTC dedifferentiation

Active Publication Date: 2020-12-18
北京科途医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after DTC metastasizes, some DTCs will dedifferentiate and develop into dedifferentiated thyroid cancer.
[0004] Dedifferentiated and undifferentiated thyroid cancer cells do not have the ability to uptake iodine, so 131 I radio

Method used

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  • Method for culturing dedifferentiated or undifferentiated thyroid carcinoma organ, and thyroid carcinoma culture medium
  • Method for culturing dedifferentiated or undifferentiated thyroid carcinoma organ, and thyroid carcinoma culture medium
  • Method for culturing dedifferentiated or undifferentiated thyroid carcinoma organ, and thyroid carcinoma culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example demonstrates the culture of undifferentiated thyroid carcinoma organoids.

[0049] (1) Preservation and transportation of undifferentiated thyroid cancer tissue samples

[0050] The puncture tissues of patients with clinically confirmed anaplastic thyroid carcinoma were obtained as anaplastic thyroid carcinoma tissue samples. Undifferentiated thyroid cancer tissue samples were stored in thyroid cancer medium and transported to the operating room within 48 hours of cold chain at 4 degrees.

[0051] (2) Obtaining undifferentiated thyroid cancer tissue cells

[0052] Place the cleaned undifferentiated thyroid carcinoma tissue sample in a sterile petri dish, add the enzymatic hydrolysis solution (DMEM / F12 containing 2 volume % FBS and 2 mg / ml collagen hydrolytic enzyme) after filtration sterilization and warm bath at 37 °C culture medium), and use sterile instruments to cut the undifferentiated thyroid cancer tissue sample into minced shape to obtain the enzy...

Embodiment 2

[0058] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 5 mM, and the concentration of BM-Cyclin was 5 μg / ml.

Embodiment 3

[0060] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 20 mM, and the concentration of BM-Cyclin was 30 μg / ml.

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Abstract

The invention relates to a method for culturing a dedifferentiated or undifferentiated thyroid carcinoma organ. The method comprises the following steps: mixing dedifferentiated or undifferentiated thyroid carcinoma tissue cells with a thyroid carcinoma culture medium and matrix glue to obtain a to-be-cultured substance, wherein the thyroid carcinoma culture medium contains nicotinamide and BM-Cyclin, and based on the thyroid carcinoma culture medium, the concentration of nicotinamide is 5-20 mM, and the concentration of BM-Cyclin is 5-30 [mu]g/ml; and carrying out culture amplification on theto-be-cultured substance to obtain the dedifferentiated or undifferentiated thyroid carcinoma organ. In the method for culturing the dedifferentiated or undifferentiated thyroid carcinoma organ, theadopted thyroid carcinoma culture medium contains nicotinamide and BM-Cyclin with specific concentrations, so that the in-vitro growth of the dedifferentiated or undifferentiated thyroid carcinoma organ is promoted. Therefore, when the method provided by the invention is used for culturing the dedifferentiated or undifferentiated thyroid carcinoma organ, the culture success rate is relatively high.

Description

technical field [0001] The present disclosure relates to the technical field of biomedicine, and in particular to a method for culturing undifferentiated or undifferentiated thyroid cancer organoids, a thyroid cancer medium for culturing undifferentiated or undifferentiated thyroid cancer organoids, and undifferentiated or undifferentiated thyroid cancer organoids. Thyroid cancer organoids. Background technique [0002] Thyroid cancer is a malignant tumor originating from thyroid follicular and parafollicular epithelial cells, including differentiated thyroid cancer and undifferentiated thyroid cancer. [0003] Differentiated thyroid carcinoma (DTC) accounts for 90% of all thyroid cancers, including papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC). DTC tumor cells have the iodine uptake ability of normal thyroid cells, which can be combined with surgical resection 131 I radiotherapy and thyroid hormone instead of suppressive therapy, so the prognosi...

Claims

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Application Information

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IPC IPC(8): C12N5/09
CPCC12N5/0617C12N5/0693C12N2500/32C12N2500/38C12N2501/11C12N2513/00
Inventor 李程黄思颖李堃孙志坚康平
Owner 北京科途医学科技有限公司
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