Cisplatin-resistant lung cancer organoid, culture method, culture medium and use thereof

Abstract

The present disclosure relates to a method for culturing cisplatin-resistant lung cancer organoids, the method comprising mixing cisplatin-resistant lung cancer tissue cells with medium and Matrigel to obtain a culture to be cultured, wherein the medium contains matrix metals Protease-10, based on the medium, the concentration of the matrix metalloproteinase-10 is 50-200nM; the culture to be cultured is cultured and expanded to obtain cisplatin-resistant lung cancer organoids. In the method for culturing cisplatin-resistant lung cancer organoids provided by the present disclosure, since the medium used contains a specific concentration of matrix metalloproteinase-10, the specific concentration of matrix metalloproteinase-10 can promote the formation of cisplatin-resistant lung cancer organoids. In vitro growth of organs. Therefore, when using the method provided by the present disclosure to culture cisplatin-resistant lung cancer organoids, the success rate of culture is relatively high.

Description

technical field [0001] The disclosure relates to the technical field of biomedicine, and in particular to a method for cultivating cisplatin-resistant lung cancer organoids, a medium for culturing cisplatin-resistant lung cancer organoids, cisplatin-resistant lung cancer organoids and their use in drugs. filter in the application. Background technique [0002] Cisplatin, also known as cis-dichlorodiammine platinum, is a widely used anti-cancer chemotherapy drug in clinical practice. It has broad-spectrum anti-tumor properties. It has a good therapeutic effect in the treatment of tumor diseases such as gastric cancer and bladder cancer. However, in the process of using cisplatin for chemotherapy, cisplatin resistance will occur, which will easily lead to the failure of chemotherapy, which greatly limits the use of cisplatin in tumor chemotherapy. [0003] Lung cancer is a common malignant tumor in the world, and its death toll accounts for about 18.4% of all cancer deaths. ...

AI Technical Summary

Problems solved by technology

Drug concentration escalation and continuous action method usually takes 6 months or more to induce drug resistance, which takes a long time, and the mode of action of its chemotherapy drugs is significantly different from the principle of high-dose and short-course chemotherapy in clinical practice

The high-dose drug intermittent induction method induces drug resistance when the drug concentration is high, and the cells are often difficult to tolerate the sudden change of the culture environment, the induction success rate is low, and the cell state is difficult to control, and the drug resistance is unstable.

In addition, the drug-resistant cell lines induced by these two methods have obvious differences in cell morphology and biological characteristics, and belong to different cell sublines, resulting in the data obtained from drug screening in the two drug-resistant cell lines are often very different.