A kind of dual-source humic acid biological fertilizer and its preparation method and application
A bio-fertilizer and humic acid technology, applied in the field of agricultural biology, can solve the problems of failure to popularize and apply, limited control effect, etc., achieve strong salt-alkali tolerance, increase yield and quality, and reduce the occurrence of rice sheath blight
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Embodiment 1
[0030] Embodiment 1: Screening of Qingsheng bacillus PAPM-10
[0031] (1) Screening of saline-alkali-tolerant strains
[0032] Qingsheng Bacillus PAPM-10 was isolated from rice rhizosphere soil in July 2018. The soil was sampled in Bincheng District, Binzhou City, Shandong Province, which is moderate saline-alkali soil. The specific separation method is as follows: mix the soil sample evenly, weigh 5g, put it into a conical flask filled with 95mL sterile water and 10 glass beads, shake it at 37°C and 180rpm for 30min. Take 1 mL of soil suspension for 10 -1 -10 -7 Serial concentration gradient dilution, and then take 10 -5 、10 -6 、10 -7 Spread the three dilutions on the plate containing the selection medium, and incubate at 37°C for 48-72h. Single colonies were picked and streaked again onto a plate containing selection medium, and cultured upside down at 37°C for 2-4 days. Then, pick a single colony and transfer it to the slant of the preservation medium test tube, cul...
Embodiment 2
[0048] Embodiment 2: Identification of Qingsheng bacillus PAPM-10
[0049] (1) Morphological and physiological and biochemical characteristics
[0050] The morphological characteristics of the Qingsheng Bacillus PAPM-10 strain: cultured on NA medium at 37°C for 48h, the colony was round and raised, and the colony was moist, wrinkled, opaque, and milky white; the thalline was rod-shaped, such as figure 1 shown. The NA medium is nutrient agar medium, and its formula is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH 7.0.
[0051] Physiological and biochemical characteristics of the Qingsheng Bacillus PAPM-10 bacterial strain: Gram-positive bacillus; positive catalase test, positive citrate utilization test, negative V-P test, positive gelatin hydrolysis test, positive starch hydrolysis test , D-glucose fermentation test was positive, D-mannitol fermentation test was positive, lactose fermentation test was positive, sucrose fermentation test was positive.
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Embodiment 3
[0054] According to the analysis of morphology, physiological and biochemical characteristics and 16S rDNA sequence, the strain is Bacillus qingshengii, named Bacillus qingshengii PAPM-10. The strain has been deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on June 22, 2020, and the preservation number is: CGMCC No. 20120. Beneficial function of embodiment 3 Qingsheng bacillus PAPM-10
[0055] (1) Ability to produce protease
[0056] Inoculate Qingsheng Bacillus PAPM-10 strain on the skimmed milk powder medium plate with a sterilized toothpick, incubate at a constant temperature of 37°C for 48-72h, measure the diameter d of the colony and the diameter D of the transparent circle, and calculate the HC value (D / d). Qingsheng Bacillus PAPM-10 strain produces proteolytic transparent circle diameter of 26.26mm, colony diameter of 10.32mm, and HC value of 2.54 on skimmed milk powder medium, which shows that the st...
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