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Nano photothermal therapy drug and preparation method thereof

A nano-drug and thermal treatment technology, applied in the field of medicine, can solve problems such as reducing the concentration of effective materials, and achieve the effects of reducing liver toxicity, easy product availability, and good biocompatibility

Pending Publication Date: 2021-01-05
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as an exogenous material, polydopamine material will cause recognition by the immune system and be quickly cleared when it enters the human body, resulting in a greatly reduced concentration of the effective material reaching the tumor site.

Method used

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  • Nano photothermal therapy drug and preparation method thereof
  • Nano photothermal therapy drug and preparation method thereof
  • Nano photothermal therapy drug and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The synthesis of embodiment 1 polydopamine nanosphere (PDA)

[0060] Add 50 mg of dopamine hydrochloride and 250 μL of concentrated ammonia water with a concentration of 25% (v / v) in 25 mL of deionized water, react for 20 h to 28 h at room temperature at a speed of 500 rpm, then centrifuge at 12000 rpm for 10 min, collect the precipitate, and use deionized Water was repeatedly washed and centrifuged to collect the precipitate until the supernatant was clear and transparent, and the precipitated product was polydopamine nanospheres.

Embodiment 2

[0061] The preparation of the polydopamine nano drug system (PDA / HI) of embodiment 2 load heat shock protein inhibitor

[0062] 6 mg of heat shock protein inhibitor was dissolved in 1 mL of organic solution DMSO to obtain a heat shock protein inhibitor solution. Disperse 7 mg of the polydopamine nanospheres obtained in Example 1 in 9 mL of deionized water to obtain a polydopamine nanosphere solution. The two solutions were mixed with a solution volume ratio of 9:1 and a solute mass ratio of 7:6. Stirred at 25°C and 300rpm for 24h, centrifuged at 12000rpm for 10min, and collected the polydopamine nanomedicine system loaded with heat shock protein inhibitors.

Embodiment 3

[0063] Example 3 Extraction of Cancer Cell Membrane (CCM)

[0064] Cell membranes were extracted using a cell membrane protein and cytoplasmic protein extraction kit. In order to avoid changes of membrane proteins caused by proteases contained or adsorbed on the membrane, the following operations should be performed under ice bath conditions.

[0065] In order to obtain cell membrane fragments, mouse breast cancer cells 4T1 were cultured in a cell culture dish with a diameter of 25 cm, and then the cells were collected with a cell scraper, centrifuged at 700 g for 5 min to obtain cell pellets, and resuspended in pre-cooled 1 ×PBS (0.01M, pH=7.4), centrifuge at 700g for 5min, and resuspend the obtained cell pellet in hypotonic cell lysate (including membrane protein extraction reagent A in the cell membrane protein and cytoplasmic protein extraction kit) and 1mM PMSF, the dosage is 20 million to 50 million cells are lysed with 990 μL cell membrane extraction reagent + 10 μL PM...

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Abstract

The invention discloses a nano photothermal therapy drug and a preparation method thereof. The nano photothermal therapy drug is composed of a nano-drug particle inner core and an outer cancer cell membrane, wherein the nano-drug system is composed of a heat shock protein inhibitor and polydopamine. The medicine can play roles in cancer cell membrane immune escape and tumor site self-targeting, photothermal therapy of polydopamine and sensitization low-temperature photothermal therapy of a heat shock protein inhibitor, carry out strike therapy for the thermal resistance of tumor cells, quicklyrelease the heat shock protein inhibitor and reduce the hepatotoxicity of the heat shock protein inhibitor, has excellent photothermal imaging and fluorescence imaging characteristics, and can be used for cancer targeted imaging and phototherapy; and high-accumulation and low-dose HI formed at self-targeting tumor sites can achieve an ideal effect, and possible toxicity to healthy organs due to excessive use is avoided.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a nano photothermal therapy drug and a preparation method thereof. Background technique [0002] Statistics show that cancer has surpassed heart disease as the leading cause of death worldwide. The current cancer treatment methods are mainly surgery, radiotherapy and chemotherapy, but these three treatments will kill normal cells while treating cancer, damage the immune system and increase the risk of secondary cancer. Photothermal therapy mainly relies on various synthetic materials (such as gold nanorods, carbon nanomaterials, copper sulfide, palladium nanosheets, near-infrared fluorescent dyes, polymers, etc.) to absorb near-infrared light and convert it into heat to kill tumor cells. . Photothermal therapy has gradually become an important technology in cancer treatment due to its advantages of high selectivity, low invasiveness, simple operation, fast recovery, and few co...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/34A61K49/00A61P35/00B82Y5/00B82Y40/00A61K31/395
CPCA61K41/0052A61K31/395A61K47/34A61K49/0019A61P35/00B82Y5/00B82Y40/00A61K2300/00
Inventor 朱静宜薛巍霍聪敏施云峰陈理恒罗司曼
Owner JINAN UNIVERSITY
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