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Yak collagen peptide and application thereof

A collagen peptide and collagen technology, applied in yak collagen peptide and its application field, can solve the problems that imported collagen peptide occupies market share, is not as popular as fish source collagen peptide, and has a low market position, so as to achieve self-improvement Effects of metabolism and repair ability, improvement of SOD activity, and promotion of proliferation

Inactive Publication Date: 2021-01-15
青海瑞肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the development of anti-aging collagen peptide products in the current market, most products are still based on fish skin collagen peptides. Among the published patents such as CN 105055188 A, CN 201010553485.9, CN 200910230201.X, CN102462640 B, etc. are selected Fish-derived collagen peptides have anti-aging effects as raw materials or added ingredients, and most merchants in the market over-promote fish skin peptides for profit, misleading consumers, resulting in a lower market position for bovine-derived collagen peptides, which are not as good as Fish-derived collagen peptides are popular, and imported collagen peptides occupy a large domestic market share

Method used

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  • Yak collagen peptide and application thereof
  • Yak collagen peptide and application thereof
  • Yak collagen peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A yak collagen peptide, the specific steps of its preparation method are as follows:

[0025] (1) Pretreatment: Weigh fresh yak hide, chop it into 1×1cm blocks after depilation, add soda ash with a mass concentration of 5% at room temperature for 8 hours at a material-to-liquid mass ratio of 1:5kg / kg, and soak for 8 hours. After the end, wash the yak skin with water until it is neutral, and then add the yak skin to the mass concentration of 5% salt at a mass ratio of 1:5kg / kg and soak it again for 8 hours. to achieve the purpose of degreasing and removing impurities;

[0026] (2) Homogenization: add 0.05M acetic acid solution into 0.05M acetic acid solution with colloid mill to obtain yak hide homogenate according to the mass ratio of material to liquid after pretreatment;

[0027] (3) Enzymolysis:

[0028] The first enzymatic hydrolysis: Add 1.5g of pepsin with an enzyme activity of 100,000 to the yak hide slurry obtained by homogenizing in step (2), and then heat it...

Embodiment 2

[0039] The effect of yak yak collagen peptide on the proliferation of human fibroblasts was detected by CCK8 method.

[0040] Human fibroblast HFF-1 in DMEM medium (containing 10% fetal bovine serum) in 5% CO 2 , cultured in a carbon dioxide incubator with a constant temperature of 37°C. Take the fibroblasts in the exponential growth phase after subculture, and adjust the cell density to 2.5×10 4 cells / mL, inoculated in 96-well plate, 100 μl per well, cultured in 37°C, 5% CO2 incubator for 24 hours, then added the sample (the sample was dissolved in DMEM culture solution and filtered with a 0.22 μm filter) to make the final concentration Reached 0.312, 3.12, 12.5mg / mL. After continuing to cultivate for 48 hours, aspirate the culture solution and add 100 μl of 10% CCK8, place in a carbon dioxide incubator and culture for 2 hours, use a multi-functional microplate reader to detect the absorbance (OD value) at 450 nm, and finally calculate the cell survival rate of each group. ...

Embodiment 3

[0043] After the yak collagen peptide of the present invention treats the fibroblasts, the intracellular SOD activity is measured.

[0044] After the fibroblasts are treated with the yak collagen peptide of the present invention, a superoxide dismutase (SOD) activity detection kit (Beijing Suolaibao Technology Co., Ltd.) is selected for the determination of intracellular SOD activity.

[0045]Human fibroblast HFF-1 in DMEM medium (containing 10% fetal bovine serum) in 5% CO 2 , cultured in a carbon dioxide incubator with a constant temperature of 37°C. Take the fibroblasts in the exponential growth phase after subculture, and adjust the cell density to 1×10 6 cells / mL, seeded in a six-well plate, 100 μl per well, at 37°C, 5% CO 2 After culturing for 24 hours in an incubator, add samples (the samples were dissolved in DMEM culture solution and filtered with a 0.22 μm filter) so that the final concentrations reached 0.312, 3.12, and 12.5 mg / mL. After 48 h of culture, the dige...

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Abstract

The invention discloses yak collagen peptide and an application thereof. The collagen peptide is high-quality Qinghai yak peptide of 3000 Da or lower, has good anti-aging and anti-oxidation effects, can be directly absorbed by a human body and can achieve good beauty and health care effect, and a beauty and health care product prepared from the collagen peptide has good anti-aging effect.

Description

technical field [0001] The invention belongs to the field of beauty care and health care, and in particular relates to a yak collagen peptide and an application thereof. Background technique [0002] With the rapid development of people's quality of life and economic level, the pursuit of beauty and the maintenance of body vitality are also increasing day by day, especially in anti-aging, which has always maintained a great demand and desire. Collagen in the body can be said to be the scaffold of all life, and plays an important role in aging. With the growth of age and the pressure of life, the soluble collagen in the body gradually decreases, causing the body to age and wrinkle. On the other hand, the growth of age, If the activity of SOD in the body decreases, the ability to scavenge harmful free radicals in the body will be reduced, which will further aggravate the aging of the body. However, a large number of studies have proved that safe and reliable anti-aging effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23J1/10A23J3/34A61K8/65A61Q19/08
CPCA23L33/18A23J1/10A23J3/342A61K8/65A61Q19/08A23V2002/00A23V2200/30A23V2200/302A23V2200/318A23V2250/55
Inventor 黄彪雷绍南
Owner 青海瑞肽生物科技有限公司
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