Plant-derived cytosine deaminases and application thereof to base editing system

Abstract

The invention discloses plant-derived cytosine deaminases and a method and application thereof to a base editing system. Experiments prove that the plant-derived cytosine deaminase can successfully perform base editing on receptor genes, and has important value for application of gene editing to plants.

Description

technical field [0001] The invention belongs to the technical field of application of genes, and specifically relates to the gene editing of plants in the field of biotechnology, using plant-derived cytosine deaminase instead of non-plant-derived cytosine deaminase in a base editing system Applications. Background technique [0002] As one of the rapidly developing biotechnologies in recent years, gene editing technology has been widely used in animals, plants and microorganisms, and the exploration of the principles of this technology or the expansion of its applications are also being actively carried out. Gene editing technology is to replace, excise, add or insert exogenous DNA sequences to a nucleotide sequence or even a single nucleotide sequence of the target gene in the cell genome to produce heritable changes. [1]- [3] Although the principles and modes of action of various gene editing technologies are different, what they have in common is that gene editing is b...

AI Technical Summary

Problems solved by technology

There are many studies on CD in plants, but there has never been any research on the application of CD from plants in gene editing technology, and there is no CD from plants at home and abroad. Reports on the application of gene editing technology

In the study of the cytosine deaminase (APOBEC) family, it was found that the functions of each protein in the APOBEC family are very diverse. Although these proteins may be classified into one category in terms of protein structure, for each APOBEC For proteins, there are large differences in their function and tissue-specific expression