Application of delphinium syringa or delphinium syringa extract in controlling mealybug of sisal pineapple
A new technology of pineapple mealybug and delphinium sacrum, applied in the direction of application, alkaloids, alkaloids, etc., to achieve the effects of environmental friendliness, long action cycle, and prevention of drug resistance
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Embodiment 1
[0029] The preparation of embodiment 1 sac from delphinium methanol extract
[0030] Grind the whole delphinium sacrum, add anhydrous methanol according to the ratio of material to liquid 1g: 10mL, seal and extract, leaching 3 times for 48 hours each time, combine the extracts, evaporate at 50°C, concentrate to extract shape to get saccharomyces from delphinium methanol extract.
Embodiment 2
[0031] The preparation of the delphinium extract of embodiment 2
[0032] 1. Preparation of Delphinium delphinium petroleum ether extract
[0033] Take the methanol extract of delphinium sacrum obtained in Example 1, suspend it in 150 times distilled water, add petroleum ether for extraction according to the volume ratio of methanol extract aqueous solution: petroleum ether = 1:2, the extraction temperature is 28 °C, The time is 2 hours, liquid separation, 50 ° C rotary evaporation, concentrated to extract-like to obtain the delphinium delphinium petroleum ether extract.
[0034] 2. Preparation of chloroform extract from delphinium
[0035] Take the methanol extract of delphinium delphinium obtained in Example 1, suspend in 150 times the volume of distilled water, add chloroform to extract according to the volume ratio of methanol extract aqueous solution: chloroform = 1:2, and the extraction temperature is 30°C for 2h, liquid separation, rotary evaporation at 45°C, and conc...
Embodiment 3
[0042] Example 3 Toxicicidal Activity Determination of Delphinium japonica Extracts to Pneumococcus Pneumococcus Pneumoniae
[0043] 1. Experimental method
[0044] Dilute the delphinium delphinium extract prepared in Example 2 with 0.1% Tween-80 aqueous solution to prepare concentrations of 10000 mg / L and 5000 mg / L. The 2nd and 3rd instar sisal pineapple gray mealybug used a concentration of 5000 mg / L of the methanol extract from the delphinium, and the concentration of the methanol extract used by the female adult was 10000 mg / L; the 2nd, 3rd, and The concentrations of the delphinium sycifera extract and the total alkaloids used by the female adult sisal cinerea cinerea were both 5000 mg / L.
[0045] Cut the fresh sisal leaves into 2cm segments, and gently sweep the same batch of nymphs or adults raised on the pumpkin onto the sisal leaves with a small hairbrush. Then sweep the worms into the corresponding plant extract or contrast solvent and soak for 5s, then take them ou...
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