The invention further discloses application of Delphinium brunonianum Royle or Delphinium brunonianum Royle extract in prevention and treatment of Dysmicoccus neobrevipes of sisal hemp

A new technology of pineapple mealybug and delphinium cysticus, applied in the fields of application, alkaloids, alkaloids, etc., to achieve the effects of environmental friendliness, high-efficiency poisoning effect, and prevention of drug resistance

Active Publication Date: 2021-01-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Mainly distributed in Tibet

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  • The invention further discloses application of Delphinium brunonianum Royle or Delphinium brunonianum Royle extract in prevention and treatment of Dysmicoccus neobrevipes of sisal hemp

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Embodiment 1

[0029] The preparation of embodiment 1 sac from delphinium methanol extract

[0030] Grind the whole delphinium sacrum, add anhydrous methanol according to the ratio of material to liquid 1g: 10mL, seal and extract, leaching 3 times for 48 hours each time, combine the extracts, evaporate at 50°C, concentrate to extract shape to get saccharomyces from delphinium methanol extract.

Embodiment 2

[0031] The preparation of the delphinium extract of embodiment 2

[0032] 1. Preparation of Delphinium delphinium petroleum ether extract

[0033] Take the methanol extract of delphinium sacrum obtained in Example 1, suspend it in 150 times distilled water, add petroleum ether for extraction according to the volume ratio of methanol extract aqueous solution: petroleum ether = 1:2, the extraction temperature is 28 °C, The time is 2 hours, liquid separation, 50 ° C rotary evaporation, concentrated to extract-like to obtain the delphinium delphinium petroleum ether extract.

[0034] 2. Preparation of chloroform extract from delphinium

[0035] Take the methanol extract of delphinium delphinium obtained in Example 1, suspend in 150 times the volume of distilled water, add chloroform to extract according to the volume ratio of methanol extract aqueous solution: chloroform = 1:2, and the extraction temperature is 30°C for 2h, liquid separation, rotary evaporation at 45°C, and conc...

Embodiment 3

[0042] Example 3 Toxicicidal Activity Determination of Delphinium japonica Extracts to Pneumococcus Pneumococcus Pneumoniae

[0043] 1. Experimental method

[0044] Dilute the delphinium delphinium extract prepared in Example 2 with 0.1% Tween-80 aqueous solution to prepare concentrations of 10000 mg / L and 5000 mg / L. The 2nd and 3rd instar sisal pineapple gray mealybug used a concentration of 5000 mg / L of the methanol extract from the delphinium, and the concentration of the methanol extract used by the female adult was 10000 mg / L; the 2nd, 3rd, and The concentrations of the delphinium sycifera extract and the total alkaloids used by the female adult sisal cinerea cinerea were both 5000 mg / L.

[0045] Cut the fresh sisal leaves into 2cm segments, and gently sweep the same batch of nymphs or adults raised on the pumpkin onto the sisal leaves with a small hairbrush. Then sweep the worms into the corresponding plant extract or contrast solvent and soak for 5s, then take them ou...

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Abstract

The invention belongs to the technical field of tropical crop pest control, and particularly relates to an application of a Delphinium brunonianum Royle or a Delphinium brunonianum Royle extract in control of Dysmicoccus neobrevipes of sisal hemp. According to the invention, the researcher finds that the Delphinium brunonianum Royle extract has an efficient poisoning effect on the Dysmicoccus neobrevipes; the Delphinium brunonianum Royle extract belongs to a plant source component, can effectively prevent the generation of drug resistance of the Dysmicoccus neobrevipes of sisal hemp, and is non-toxic and harmless to human and livestock, environment-friendly and relatively long in action period. According to the invention, a green and safe choice is provided for the development of botanicalpesticides for preventing and controlling the Dysmicoccus neobrevipes of sisal hemp.

Description

technical field [0001] The invention belongs to the technical field of tropical crop pest control. More specifically, it relates to the application of the delphinium or delphinium extract in the prevention and treatment of sisal pineapple mealybug. Background technique [0002] The new pineapple mealybug is an important pest on sisal, mainly harming pineapple, banana, custard apple, citrus, grape and other plants. Eggs, larvae and adults all parasitize on the surface of host plants, which can cause malnutrition of host plants, weakened tree vigor, a large number of leaf and fruit drop, and cause plant death in severe cases. The female sucks mesophyll juice for a living, and the pest also secretes honeydew, which causes a large number of soot diseases, seriously affects the photosynthesis of plants, and leads to a decline in the economic value of plants. The insect is widely distributed in tropical and subtropical regions. Once spread, it will cause serious damage to fruits...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/32A01P7/04C07G5/00
CPCA01N65/00A01N65/32C07G5/00
Inventor 曾鑫年郑丹杰刘家莉
Owner SOUTH CHINA AGRI UNIV
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