A kind of 3d culture medium of brain glioma organoid and its application

A technology of glioma and organoids, which is applied in the field of biomedicine, can solve the problems of colon, stomach, prostate and pancreas and other tissues that have been successfully constructed in vitro in organ culture, so as to avoid hypoxic damage, accelerate proliferation, The effect of promoting cluster growth
CN112300996BActive Publication Date: 2021-06-25ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
Publication Date
2021-06-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention provides a 3D culture medium for glioma organoids and its application. The culture medium includes basal medium and specific additive factors; the specific additive factors include the following final concentration components: B27 additive, 1 L-Glutamine, 0.05-0.2%; HGF, 10-50ng / ml; Insulin, 0.03-0.06%; FGF-2, 10-50ng / ml; dorsomorphin, 2-3μM; SB‑431542, 20‑30μM; EGF, 100‑500ng / ml; HEPES, 10‑30mM; BSA, 1~10%; Heparin, 0.01‑0.1%; Human NLGN3, 50‑200nM; %; the aforementioned percentage concentration represents the mass concentration. By adopting the culture medium of the present invention to cultivate glioma organoids, the glioma tissue cells can better exhibit their inherent activity characteristics during the culture process, and achieve comprehensive characteristics highly similar to living brain tissues.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a 3D medium for glioma organoids and an application thereof. Background technique

[0002] Glioma is a common malignant tumor of the central nervous system, accounting for about 40%-50% of intracranial tumors, mostly in the form of infiltrative growth, with unclear boundaries with the surrounding brain tissue, and difficult to be completely removed by surgery. It is easy to relapse after surgery; generally speaking, the higher the grade of the tumor, the higher its malignancy, and the faster the recurrence after surgery. At present, the treatment of glioma is mainly based on surgical resection combined with postoperative radiotherapy and chemotherapy, but the prognosis is poor. In recent years, no breakthrough has been made in the treatment of glioma.

[0003] However, currently, there are only a handful of in vitro glioma models used in drug development and bas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More