Kit for detecting rat peripheral blood HDAC5 expression level, and use method of kit

A technology of expression level and peripheral blood, applied in the field of peripheral blood detection, can solve problems such as cumbersome operation, and achieve the effect of highlighting substantive characteristics

Pending Publication Date: 2021-02-09
河南省精神病医院
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the operation is cumbersome when detecting the expression level of HDAC5. Therefore, there is an urgent need for a kit that can detect the expression level of HDAC5 in rat peripheral blood mononuclear cells.

Method used

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  • Kit for detecting rat peripheral blood HDAC5 expression level, and use method of kit
  • Kit for detecting rat peripheral blood HDAC5 expression level, and use method of kit
  • Kit for detecting rat peripheral blood HDAC5 expression level, and use method of kit

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Embodiment

[0040] A kit for detecting the expression level of HDAC5 in peripheral blood of rats, the reagents contained in the kit and instructions for use are shown in the following table:

[0041]

[0042] In the table: Primer Ⅰ: GTCGAAAGGATGGCACTGTT (upstream of HDAC5)

[0043] Primer II: AGCCAGTAAAGCCGTTCTCA (downstream of HDAC5)

[0044] Primer III: GGAGCGAGATCCCGTCAAGA (upstream of GAPDH)

[0045] Primer IV: CACAAACATGGGGGCATCAG (downstream of GAPDH).

[0046] A method for using the above-mentioned kit, which includes the following operation process:

[0047] Step 1: Isolation of Peripheral Blood Mononuclear Cells

[0048] Take 5mL of heparin anticoagulant blood, drip slowly along the tube wall and superimpose it into a test tube containing 5mL of solution a. Care should be taken to keep the interface between the two clear, centrifuge at 2000r / min for 20min at room temperature, and gently suck out with a capillary pipette. Milky white mononuclear cells;

[0049] Step 2: Ext...

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Abstract

The invention provides a kit for detecting a rat peripheral blood HDAC5 expression level, and a use method of the kit. Through the kit, a pregnant infection adolescent offspring rat of which the peripheral blood monocyte HDAC5 expression quantity is obviously up-regulated is screened to be continuously raised to an adult stage to carry out behavior detection (an open field test, a Y maze experiment and prepulse inhibition (PPI)). A result shows that the adult stage of the rat of which the HDAC5 obviously rises is obviously abnormal, for example, an anxiety level is increased, memory deficits are in the presence, sensory gating dysfunctions are in the presence and the like. Therefore, on the basis of the above inference, the kit can effectively evaluate the model quality of a pregnant infection adolescent offspring and can be used for subsequent researches.

Description

technical field [0001] The invention belongs to the technical field of peripheral blood detection, and in particular relates to a kit for detecting the expression level of HDAC5 in peripheral blood of rats and a use method thereof. Background technique [0002] By preparing the offspring rat model infected during pregnancy, the expression levels of HDAC5 in the hippocampus, prefrontal cortex and peripheral mononuclear cells of puberty (35 days after birth) and adulthood (56 days after birth) were detected, combined with behavioral analysis, it was found that The increase of HDAC5 in peripheral blood mononuclear cells is earlier than the appearance of abnormal behavior. Therefore, HDAC5 can be used as an early peripheral marker molecule to evaluate the quality of the offspring rat model infected during pregnancy. However, it is cumbersome to detect the expression level of HDAC5. Therefore, there is an urgent need for a kit that can detect the expression level of HDAC5 in rat ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2531/113C12Q2545/114C12Q2521/107C12Q2561/113
Inventor 李文强苏玺宋盟邵明龙刘青张露文吕路线
Owner 河南省精神病医院
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