T-DNA flanking sequence of transgenic saccharum SCAG2 and transformation event specificity identification method of T-DNA flanking sequence

Abstract

The invention discloses a T-DNA flanking sequence of transgenic saccharum SCAG2 and a transformation event specificity identification method of the T-DNA flanking sequence. The T-DNA flanking sequenceof the transgenic saccharum SCAG2 comprises a left flanking sequence shown as a nucleotide sequence SEQ ID NO. 1 and / or a right flanking sequence shown as a nucleotide sequence SEQ ID NO. 2. The leftand right flanking sequences of a transgenic saccharum SCAG2 exogenous gene insertion site are obtained through amplification for the first time, the event specificity detection method of the transgenic saccharum SCAG2 is established on the basis of the sequences, the method is good in specificity and high in sensitivity, and when the relative content of transgenic ingredients is 0.1% or above, aconventional qualitative PCR method can be used for detection; The sensitivity is far higher than the lowest limit of 0.9% of the identification of European Union on transgenic plant-derived foods and feeds, and a key technical basis is provided for the utilization of the transgenic saccharum SCAG2 and product detection.

Description

technical field [0001] The invention relates to the field of transgenic sugarcane, in particular to a T-DNA flanking sequence of transgenic sugarcane SCAG2 and a specific identification method for transformation events thereof. Background technique [0002] Sugarcane (Saccharum spp.) is the fifth largest crop in the world and the most important sugar crop and biofuel crop in the world. Data from the China Sugar Industry Association shows that in 2020, 1.191 million hectares of sugarcane will be planted across the country, with a sugar production of 8.419 million tons and an annual direct agricultural output value of about 48 billion yuan. Therefore, the sugarcane industry is of great significance to the national economy. However, the damage caused by various borers on sugarcane seriously affects the yield and quality of sugarcane. Taking the Hunan-Guangxi sugarcane area as an example, from 2018 to 2019, sugarcane pests in this area were extremely serious, with an average pla...

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Problems solved by technology

However, the damage caused by various borers in sugarcane seriously affects the yield and quality of sugarcane. Taking the Xianggui sugarcane area as an example, from 2018 to 2019, sugarcane pests in this area were extremely serious, with an average plant rate of borer damage as high as 46.54%. The loss is 14.87%, the average sucrose loss is as high as 1.12 percentage points, the agricultural loss is 467 million yuan, the industrial loss is 856 million yuan, and the national financial loss is 51 million yuan

Therefore, cultivating new insect-resistant sugarcane germplasm has always been an important goal of sugarcane breeding. However, sugarcane is a highly heterozygous vegetative crop, and its complex genetic background, which brings a lot of blindness to the breeding work

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