Method, system and device for automatic stitching of microscopic sequence images based on affine transformation

Abstract

The invention belongs to the technical field of computer vision and image processing, and specifically relates to a method, system and device for automatically mosaicing microscopic sequence images based on affine transformation, aiming to solve the problem that the prior art cannot effectively solve the problems in the microscopic image shooting process. The influence of deformation on splicing, splicing errors will accumulate and propagate, so the splicing effect cannot meet the expected problem. The invention includes: extracting the features of the overlapping area of ​​the microsequence images and performing feature matching; assigning different weights to each pair of feature points according to the position information of the features on the image; fitting the changes between adjacent images through an affine transformation model, And set the energy function for global optimization; minimize the energy function to obtain the affine transformation relationship of each adjacent image; perform splicing according to the affine transformation relationship to obtain the microscopic mosaic image. The present invention avoids the influence of error accumulation and image edge distortion on the result in the stitching process through the method of global stitching, and can obtain images with higher stitching precision.

Description

technical field [0001] The invention belongs to the technical field of computer vision and image processing, and in particular relates to an affine transformation-based automatic splicing method, system and device for microsequence images. Background technique [0002] From the manufacture of the first optical microscope in the early 17th century to the design of the first transmission electron microscope in the early 20th century, human beings have gradually deepened their exploration of the microscopic world. With the rapid development of electronic technology, the resolution of some transmission electron microscopes can reach sub-angstrom level, and people can clearly see the structure of atoms. Microscopes are increasingly used in fields such as medical research and biology, as the images obtained are at higher and higher resolutions. It also brings a problem, the higher the resolution of the image obtained, the smaller the imaging field of view of the microscope. In t...

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Problems solved by technology

[0008] In order to solve the above-mentioned problems in the prior art, that is, the prior art cannot effectively solve the influence of the image deformation on the splicing during the microscopic image shooting process, and the splicing errors will accumulate and propagate, so the splicing effect cannot reach the expected problem. The invention provides an affine transformation-based automatic mosaic method for microscopic sequence images, the method comprising:

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