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Ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material and method

A calcium phosphate carboxymethyl chitosan, amorphous technology, applied in the field of bone implant materials to achieve the effects of promoting proliferation, good biocompatibility, and promoting bone repair

Active Publication Date: 2022-03-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People have made great efforts to explore the mineralization of collagen from the nanometer to the macroscopic scale, and to prepare composite materials with natural bone composition and structure. However, the minimum size (thickness) of hydroxyapatite inside the mineralized collagen is only 2nm, but most of the amorphous calcium phosphate in the currently prepared bone tissue engineering materials is much larger than 2nm

Method used

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  • Ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material and method
  • Ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material and method
  • Ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material and method

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Embodiment 1

[0029] In the present embodiment, an ultra-small particle diameter amorphous phosphate carboxyme-chip glycan nanoclustric carboxymethyl-glycan, the preparation method is specifically:

[0030] S1: Calcium chloride (CaCl) with a concentration of 1 m 2 The solution was slowly dropped into a polyteenine (PASP) solution of 0.15 ml of concentration of 0.3 g / ml, and stirred at 500 rpm to fully react, resulting in solution A.

[0031] During this reaction, PASP as an anionic polymer, the carboxyl group on the side chain can chelate CA 2+ To form a polyteenine salt, thereby stabilizing CA 2+ .

[0032] S2: The solution A obtained in S1 was slowly added dropwise to a carboxymethyl chitosan (CMC) solution of 2 ml of concentration of 100 mg / mL, stirred overnight at a rotational speed of 500 rpm (i.e., at least 6 h), so that it fully reacted Get solution B.

[0033] During the reaction, since the carboxymethyl chitosan (CMC) is introduced into a carboxymethyl group in chitosan, it is poss...

Embodiment 2

[0038] In order to further study Example 1 Preparation of the resulting ultra-small diameter amorphous phosphate carboxymethyl chitosan nanoclustric calcium carboxymethylge glycan nanoclustric material, the present embodiment has been carried out according to the corresponding experimental verification.

[0039] Such as figure 1 As shown, the scanning electron microscope diagram after material freezing, can be seen from the figure, the material is a three-dimensional multi-hole network structure, and its void distribution is uniform, and a uniform micrometer gap is distributed on the nanometer void, and natural cells can be simulated. The outer matrix, supporting cell migration aggregation and growth.

[0040] Such as figure 2 As shown, for the transmission electron microscope of the material solution, the upper left corner is a diffraction ring, and the white arrow is referred to as amorphous phosphate particles. As can be seen from the figure, the material solution contains abou...

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Abstract

The invention discloses an ultra-small-diameter amorphous calcium phosphate carboxymethyl chitosan nanocluster material and a method thereof. The preparation method is as follows: anhydrous calcium chloride CaCl 2 The solution was dropped into the polyaspartic acid PASP solution, stirred to make it fully react, and solution A was obtained; solution A was added dropwise to carboxymethyl chitosan CMC solution, stirred to make it fully react, to obtain solution B; Disodium Hydrogen Na 2 HPO 3 The solution is added into the solution B, stirred to make it fully react, and then the pH value of the mixed solution is adjusted to 7-8 to obtain an amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with an ultra-small particle size. The material prepared by the invention has good biocompatibility, can promote the proliferation of cells, and can regulate the polarization of macrophages, so that the macrophages develop in the direction of M2, and can promote bone repair.

Description

Technical field [0001] The present invention belongs to the field of bone implantation materials, and more particularly to ultra-small particle diameter amorphous calcium carboxymethyl chitosan nanoclutic materials and methods thereof. Background technique [0002] As the incidence of bone damage is increasing, the demand for rapid and effective repair of bone defects is increasing. Bone transplantation is currently a commonly used treatment method, but the autologous bone transplantation is limited, and allogeneic bone is easy to produce immune rejection, these limitations have stimulated the booming of bone tissue projects. [0003] Inspired by biological mineralization, the inactive component doped in the matrix material is a promising strategy. Excellent bone group weaving engineering materials are reproducible materials that can achieve recycled materials by fully simulating the characteristics of natural bone tissue matrices. Mineralized collagen fibers constitute the basic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/50
CPCA61L27/20A61L27/12A61L27/50A61L2400/12A61L2430/02A61L2400/06C08L5/08
Inventor 谢志坚周焰焰唐睿康胡秭禾姚沙沙陈卓石珏
Owner ZHEJIANG UNIV
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