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Amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size and method

A technology of calcium phosphate carboxymethyl chitosan and carboxymethyl chitosan, which is applied in the field of bone implant materials to achieve the effects of facilitating injection, regulating polarization, and temperature-sensitive fluidity

Active Publication Date: 2021-02-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People have made great efforts to explore the mineralization of collagen from the nanometer to the macroscopic scale, and to prepare composite materials with natural bone composition and structure. However, the minimum size (thickness) of hydroxyapatite inside the mineralized collagen is only 2nm, but most of the amorphous calcium phosphate in the currently prepared bone tissue engineering materials is much larger than 2nm

Method used

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  • Amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size and method
  • Amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size and method
  • Amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size and method

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Embodiment 1

[0029] In the present embodiment, a kind of ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material is prepared, and the preparation method is as follows:

[0030] S1: Dissolve 0.1mL of 1M anhydrous calcium chloride (CaCl 2 ) solution was slowly dropped into 0.15 mL of polyaspartic acid (PASP) solution with a concentration of 0.3 g / mL, stirred at a speed of 500 rpm for 10 min to fully react, and solution A was obtained.

[0031] During this reaction, PASP acts as an anionic polymer, and the carboxyl group on its side chain can chelate Ca 2+ , forming polyaspartate, thereby stabilizing Ca 2+ .

[0032] S2: Slowly add the solution A obtained in S1 to 2 mL of carboxymethyl chitosan (CMC) solution with a concentration of 100 mg / mL, and stir overnight at a speed of 500 rpm (that is, at least 6 h) to make it fully react , to obtain solution B.

[0033] In this reaction process, since carboxymethyl chitosan (CMC) introduces carboxymethyl ...

Embodiment 2

[0038] In order to further study the performance of the ultra-small particle size amorphous calcium phosphate carboxymethyl chitosan nanocluster material prepared in Example 1, corresponding experiments were carried out in this example.

[0039] Such as figure 1 Shown is the scanning electron microscope image of the material after freeze-drying. It can be seen from the figure that the material is a three-dimensional porous network structure, and its voids are evenly distributed. The nano-scale voids are distributed with uniform micron-scale voids, which can simulate natural cells. The extracellular matrix supports cell migration and growth.

[0040] Such as figure 2 Shown is the transmission electron microscope image of the material solution, the upper left corner is the diffraction ring, and the white arrow points to the amorphous calcium phosphate particles. It can be seen from the figure that the material solution contains calcium phosphate particles of about 1 nm, and t...

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Abstract

The invention discloses an amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size and a preparation method of the nano-cluster material. The preparation method comprises the steps that an anhydrous calcium chloride CaCl2 solution is dropwise added into a polyaspartic acid PASP solution and stirred for a sufficient reaction, and a solution A is obtained; the solution A is dropwise added into a carboxymethyl chitosan CMC solution, and stirring is conducted to realize sufficient reaction, and a solution B is obtained; and a disodium hydrogen phosphate Na2HPO3 solution is added into the solution B, stirring is conducted to realize sufficient reaction, and the pH value of the mixed solution is regulated to 7-8 to obtain the amorphous calcium phosphate carboxymethyl chitosan nano-cluster material with ultra-small particle size. The prepared material is good in biocompatibility, can promote cell proliferation, can adjust polarization of macrophages, enables the macrophages to develop towards M2, and can promote bone repair.

Description

technical field [0001] The invention belongs to the field of bone implant materials, and in particular relates to an ultra-small-diameter amorphous calcium phosphate carboxymethyl chitosan nano-cluster material and a method thereof. Background technique [0002] With the increasing incidence of bone damage, there is an increasing demand for rapid and effective repair of bone defects. Bone transplantation is a commonly used clinical treatment method at present, but the bone source of autologous bone transplantation is limited, and allogeneic bone is prone to immune rejection. These limitations have stimulated the vigorous development of bone tissue engineering. [0003] Inspired by biomineralization, doping inorganic components in matrix materials is a promising strategy to enhance the osteoinductive ability of hydrogels. Excellent bone tissue engineering materials are materials that can achieve regeneration by fully simulating the properties of natural bone tissue matrix. ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/12A61L27/50
CPCA61L27/20A61L27/12A61L27/50A61L2400/12A61L2430/02A61L2400/06C08L5/08
Inventor 谢志坚周焰焰唐睿康胡秭禾姚沙沙陈卓石珏
Owner ZHEJIANG UNIV
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