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Injection gel containing submicron hyaluronic acid microspheres and preparation method

A hyaluronic acid, sub-micron technology, applied in the fields of pharmaceutical formulation, medical science, prosthesis, etc., can solve the problem of reducing the elastic properties of injection gels of hyaluronic acid microspheres, poor gel shrinkage performance, and ineffective use. Good and other problems, to achieve good cell adhesion, shape maintenance, and improve the effect of use

Pending Publication Date: 2020-11-13
扬州美瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides an injection gel containing submicron-sized hyaluronic acid microspheres and a preparation method to solve the problem of injection gelation of the currently used hyaluronic acid microspheres proposed in the above-mentioned background technology. The gel particles inside the glue are relatively large, and the shrinkage performance between the gels is not good during use, which greatly reduces the elastic performance of the injection gel of hyaluronic acid microspheres, and the injection gel of hyaluronic acid microspheres is followed by The deposition of polylactic acid microspheres is gradually accelerated, resulting in local stiffness, poor use effect, and problems that cannot meet the needs of use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing an injection gel containing submicron hyaluronic acid microspheres, the preparation method of which is as follows:

[0028] Step 1: Take 3g of sodium hyaluronate and 97ml of pure water for stirring and dissolving to obtain a 3% sodium hyaluronate solution;

[0029] Step 2: Add 10L of trifluoroethanol organic solvent to the 3% sodium hyaluronate solution for mixing, and add 15g of L-lactic acid microspheres for stirring reaction. The reaction temperature is set at 65°C, and the stirring rate is set at Set at 200r / min, and the stirring time is set at 15min. After the stirring reaction is completed, the solution is subjected to electrospinning treatment. The spinning parameters are controlled at a voltage of 5KV, a nozzle aperture of 0.2mm, a solution flow rate of 0.5ml / h, and an ambient temperature of 20°C. The distance between the receiving plate and the nozzle is 5 cm, and a suspension with cross-linking function is obtained;

[0030] Step 3: Tra...

Embodiment 2

[0036] A method for preparing an injection gel containing submicron hyaluronic acid microspheres, the preparation method of which is as follows:

[0037] Step 1: Take 4g of sodium hyaluronate and 96ml of pure water for stirring and dissolving to obtain a 4% sodium hyaluronate solution;

[0038] Step 2: Add 10L of trifluoroethanol organic solvent to the sodium hyaluronate solution with a concentration of 4% for mixing, and add 20g of L-lactic acid microspheres for stirring reaction. The reaction temperature is set at 65°C, and the stirring rate is set at Set at 200r / min, and the stirring time is set at 15min. After the stirring reaction is completed, the solution is subjected to electrospinning treatment. The spinning parameters are controlled at a voltage of 5KV, a nozzle aperture of 0.2mm, a solution flow rate of 0.5ml / h, and an ambient temperature of 20°C. The distance between the receiving plate and the nozzle is 5 cm, and a suspension with cross-linking function is obtained;...

Embodiment 3

[0045] A method for preparing an injection gel containing submicron hyaluronic acid microspheres, the preparation method of which is as follows:

[0046] Step 1: Take 5g of sodium hyaluronate and 95ml of pure water for stirring and dissolving to obtain a 5% sodium hyaluronate solution;

[0047] Step 2: Add 10L of trifluoroethanol organic solvent to the 5% sodium hyaluronate solution for mixing, and add 15g of L-lactic acid microspheres for stirring reaction. The reaction temperature is set at 65°C, and the stirring rate is set at Set at 200r / min, and the stirring time is set at 15min. After the stirring reaction is completed, the solution is subjected to electrospinning treatment. The spinning parameters are controlled at a voltage of 5KV, a nozzle aperture of 0.2mm, a solution flow rate of 0.5ml / h, and an ambient temperature of 20°C. The distance between the receiving plate and the nozzle is 5 cm, and a suspension with cross-linking function is obtained;

[0048] Step 3: Tra...

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Abstract

The invention relates to the technical field of injection gel. The invention further discloses injection gel containing the submicron hyaluronic acid microspheres and a preparation method. The injection gel is prepared from sodium hyaluronate with the molecular weight of 200,000 to 3,000,000 Daltons, pure water, a trifluoroethanol organic solvent, L-polylactic acid microspheres, a 1 mol / L sodium hydrochloride solution, 100g to 200g / L adipic acid dihydrazide and 300g to 600g / L 1-hydroxybenzotriazole, 75% absolute ethyl alcohol, 2.5% normal saline, deionized water and a PBS buffer solution withthe sodium phosphate concentration of 0.1 mol / L. According to the injection gel containing the submicron hyaluronic acid microspheres and the preparation method of the injection gel, particles of theinjection gel are submicron, so that the shrinkage performance between the gel is improved, the elasticity of the injection gel is improved, the absorption time of hyaluronic acid can be greatly prolonged, the deposition of polylactic acid microspheres is reduced, and the use effect is indirectly improved.

Description

technical field [0001] The invention relates to the technical field of injection gel, in particular to an injection gel containing submicron hyaluronic acid microspheres and a preparation method thereof. Background technique [0002] Hyaluronic acid, also known as hyaluronic acid and hyaluronic acid, is a natural polysaccharide structure composed of repeated D-glucuronic acid and N-acetylglucose through β-1,3-glucosidic bonds. It exists in the interstitium of animal cells and is an important basic substance for skin suppleness. It has strong water absorption and special water retention. It is the most moisturizing substance found in nature so far. It is called an ideal natural moisturizing factor. In addition, hyaluronic acid can also improve skin nutrient metabolism, make skin soft, smooth, wrinkle-free, increase elasticity, and prevent aging. It is also a good transdermal absorption accelerator while moisturizing. To the more ideal effect of promoting nutrient absorption....

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/50A61L27/18A61L2/10
CPCA61L2/10A61L27/18A61L27/20A61L27/50A61L2400/06A61L2430/34C08L5/08C08L67/04
Inventor 林琳颜先琴陈美娟管荣健
Owner 扬州美瑞生物科技有限公司
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