Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial

A technology of biological activity and biomaterials, applied in the field of biomaterials and their preparation, and polypeptide bioactive coatings, can solve problems such as limited development and complexity, and achieve the effect of promoting cell adhesion, good cell adhesion, and promoting autoimmune function

Inactive Publication Date: 2016-04-20
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LB deposition requires complex equipment and is only suitable for amphiphilic molecules, and SAM is only suitable

Method used

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  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial
  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial
  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0051] Example 1

[0052] Follow the steps below to prepare PLL-PLGA antibacterial coating:

[0053] Use stainless steel sheet as the base material, after ultrasonic cleaning in 70% alcohol aqueous solution, immerse it in 1mg / ml PLL Glycine-NaOH (50mM, pH=10) buffer solution for 15 minutes, and wash in Glycine-NaOH solution Then air dry; immerse the sample in 1mg / ml PLGA Glycine-NaOH (50mM, pH=10) buffer solution for 15 minutes, clean and air dry; repeat the above steps 20 times to obtain 20 double layers PLL / PLGA coating: immerse the sample in a PBS solution of cephalosporin sodium (5mg / ml) for 5 minutes to obtain a polypeptide coating with antibiotics.

[0054] The ultraviolet-visible light absorption measurement of the coating in this example:

[0055] Such as figure 1 As shown, for measurement, quartz glass is used as the material, and every two layers of polypeptide coating are deposited, and the quartz plate sample is placed in an ultraviolet-visible spectrophotometer to measur...

Example Embodiment

[0060] Example 2

[0061] Follow the steps below to prepare polypeptide antibacterial and biologically active coatings

[0062] Titanium alloy metal pieces are ultrasonically cleaned in 70% alcohol aqueous solution, and then immersed in a 1mg / ml Tris-NaAc (10mM, pH=4.0) buffer solution of PLL for 10 minutes. After cleaning in the solution, air blow dry; Immerse in 0.5mg / ml Arg-Gly-Asp (RGD) Tris-NaAc buffer solution for 15 minutes, clean and air dry; immerse the sample in 1mg / ml PLGA Tris-NaAc buffer solution for 15 minutes, Air dry after washing; repeat the above steps 20 times to obtain the PLL / RGD / PLGA coating; immerse the sample in a PBS solution of gentamicin (5mg / ml) and TGF-β (0.5ug / ml) 5 Minutes, rinse and blow dry to obtain polypeptide antibacterial and biologically active coating.

[0063] The coating in this embodiment was analyzed by ultraviolet spectroscopy, in which the RGD layer was labeled with fluorescein isothiocyanate (FITC), which corresponds to the absorption p...

Example Embodiment

[0074] Example 3

[0075] Follow the steps below to prepare PLL-PLGA antibacterial coating:

[0076] Use stainless steel sheet as the base material, after ultrasonic cleaning in 70% alcohol aqueous solution, immerse it in 0.5mg / ml PLL Glycine-NaOH (50mM, pH=12) buffer solution for 20 minutes, in Glycine-NaOH solution Air dry after cleaning; immerse the sample in 0.5mg / ml PLGA Glycine-NaOH (50mM, pH=12) buffer solution for 20 minutes, and air dry after cleaning; repeat the above steps 20 times to obtain 20 double Layer of PLL / PLGA coating; immerse the sample in a PBS solution of cephalosporin sodium (5mg / ml) for 5 minutes to obtain a polypeptide coating with antibiotics.

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Abstract

The invention provides a polypeptide bioactive coating, which mainly comprises a plurality of polypeptide film layers and an active material layer, wherein two adjacent polypeptide film layers have different charges; two adjacent polypeptide films are combined through charge acting force; each polypeptide film is 3-10nm in thickness; a nano coating material of the polypeptide bioactive coating is polypeptide with good biocompatibility, not only meets the characteristics of being nontoxic, free of thrill, and free of carcinogenesis, teratogenesis and mutagenesis, but also has good bacteriostatic and antibacterial effects, has biological activity and is capable of promoting cell adhesion, growth and proliferation. The invention further provides a biomaterial, which comprises a base material and the bioactive coating loaded on the surface of the base material. The invention further provides a method for preparing the biomaterial. The invention further provides an application of the biomaterial in a surgical operation; and preferably, the surgical operation is an orthopedic operation.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a polypeptide biologically active coating, a biological material and a preparation method and application thereof. Background technique [0002] The progress of modern medicine is inseparable from the development of biomaterials. From plastic surgery, cardiovascular implantation stents, human orthopedic replacement and repair, biological tissue engineering to drug delivery systems, medical biomaterials are used in various fields of biomedicine [1]. Implantation of these materials into the human body often causes local and systemic adverse reactions. As a biomaterial, it must meet the following requirements: non-toxic, non-irritating, and non-toxic, and have good compatibility with cells and tissues. The host reaction caused by the material is microscopically caused by the host cell's recognition of the surface, so the properties of the biological surface often determine whether the m...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54A61L31/10A61L31/16
CPCA61L27/34A61L27/54A61L31/10A61L31/16A61L2300/214A61L2300/404A61L2300/406A61L2300/426A61L2300/606A61L2400/18A61L2420/08C08L77/04
Inventor 江兵兵陈学琴
Owner HUBEI UNIV
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