Active polypeptide, and preparation method and use of active polypeptide in antioxidant mask
A technology of active peptides and peptides, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low extraction purity and high activity, and achieve strong anti-oxidation ability, good skin care effect, and improved activity.
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Embodiment 1
[0053] Example 1 Preparation of active polypeptide
[0054] Pretreatment: After cutting the sturgeon skin, scrape off the fat and connective tissue under the skin, then cut it into pieces, soak it in 10wt% NaCl solution to remove soluble non-collagen protein, filter out the residue, Soak in a mixture of isopropanol, ether and distilled water in a weight ratio of 3:2:8 to remove fat, filter and dry to obtain a substrate.
[0055] The first enzymatic hydrolysis: The substrate was prepared into a 0.2wt% suspension in deionized water, and then the pH was adjusted to about 6.0 with dilute HCl, and bromelain was added. The amount of enzyme added was 2000U / g of substrate. Enzymatic hydrolysis was carried out for 90 min under conditions, then the enzyme was inactivated at 100 °C for 10 min, and then NaOH was added to adjust the pH to neutrality to obtain the first enzymatic hydrolysis product;
[0056] Large pore size ultrafiltration treatment: The first enzymatic hydrolysis product ...
Embodiment 2
[0059] Example 2 Preparation of Active Polypeptides
[0060] Pre-treatment: After cutting the sturgeon skin, scrape off the fat and connective tissue under the skin, then cut it into pieces, soak it in 12wt% NaCl solution to remove soluble non-collagen protein, filter out the residue, Soak in isopropanol, ether and distilled water in a weight ratio of 2:3:7 to remove fat, filter and dry to obtain a substrate.
[0061] The first enzymatic hydrolysis: The substrate was prepared into a 0.3wt% suspension in deionized water, and then adjusted to pH about 6.0 with dilute HCl, and bromelain was added. Enzymatic hydrolysis was carried out for 85 min under the conditions, then the enzyme was inactivated at 100 °C for 10 min, and then NaOH was added to adjust the pH to neutrality to obtain the first enzymatic hydrolysis product;
[0062] Large pore size ultrafiltration treatment: the first enzymatic hydrolysis product is filtered with an ultrafiltration membrane with a molecular weight...
Embodiment 3
[0065] Example 3 Preparation of Active Polypeptides
[0066] Pretreatment: After cutting the sturgeon skin, scrape off the fat and connective tissue under the skin, cut it into pieces, soak it in 8wt% NaCl solution to remove soluble non-collagen protein, filter out the residue, Soak in isopropanol, ether and distilled water in a weight ratio of 2:3:9 to remove fat, filter and dry to obtain a substrate.
[0067] The first enzymatic hydrolysis: The substrate was prepared into a 0.2wt% suspension in deionized water, and then the pH was adjusted to about 6.0 with dilute HCl, and bromelain was added. The amount of enzyme added was 2000U / g of substrate. Enzymatic hydrolysis was carried out for 75 min under conditions, then the enzyme was inactivated at 100 °C for 10 min, and then NaOH was added to adjust the pH to neutrality to obtain the first enzymatic hydrolysis product;
[0068] Large pore size ultrafiltration treatment: the first enzymatic hydrolysis product is filtered by an ...
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