Preparation method of functional food for balancing intestinal flora and intestinal immunity

A technology of intestinal flora and functional food, applied to the functions of food ingredients, methods based on microorganisms, biochemical equipment and methods, etc. problems, to achieve the effect of improving the structure of intestinal flora, lowering cholesterol, and balancing intestinal immunity

Active Publication Date: 2021-03-02
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is no relevant record of the

Method used

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  • Preparation method of functional food for balancing intestinal flora and intestinal immunity
  • Preparation method of functional food for balancing intestinal flora and intestinal immunity
  • Preparation method of functional food for balancing intestinal flora and intestinal immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Weigh 5g of poly-γ-glutamic acid (γ-PGA), add purified water to prepare a γ-PGA solution with a mass concentration of 10%;

[0032] (2) Weigh 5g of glucomannan (KMG), add purified water to prepare a KMG solution with a mass concentration of 5%;

[0033] (3) Add 5ml of poly-γ-glutamic acid solution dropwise to 5ml of glucomannan solution, stir well, and centrifuge at 1000r / min to remove bubbles to obtain a γ-PGA / KMG mixed solution;

[0034] (4) Divide the above mixed solution into 5ml centrifuge tubes, 4ml in each tube, then freeze at -20°C for 12h, then thaw at 8°C for 2h, cycle 3 times, put the formed complex solution in Dry in a vacuum dryer at 40°C for 9 hours to obtain composite fibers.

Embodiment 2

[0036] (1) Weigh 5g poly-γ-glutamic acid (γ-PGA), add purified water to prepare γ-PGA solution;

[0037] (2) Weigh an appropriate amount of glucomannan (KMG), and add purified water to prepare a KMG solution;

[0038] (3) Add 6ml of poly-γ-glutamic acid solution dropwise to 5ml of glucomannan solution, stir well, and centrifuge at 3000r / min to remove bubbles to obtain a γ-PGA / KMG mixed solution;

[0039] (4) Divide the above mixed solution into 5ml centrifuge tubes, 4ml in each tube, then freeze at -50°C for 12 hours, then thaw at 20°C for 3 hours, cycle 3 times, put the formed composite solution in Dry in a vacuum dryer at 40°C for 12 hours to obtain composite fibers.

Embodiment 3

[0041] (1) Weigh 5g poly-γ-glutamic acid (γ-PGA), add purified water to prepare γ-PGA solution;

[0042] (2) Weigh an appropriate amount of glucomannan (KMG), and add purified water to prepare a KMG solution;

[0043] (3) Add 8ml of poly-γ-glutamic acid solution dropwise to 8ml of glucomannan solution, stir well, and centrifuge at 2000r / min to remove bubbles to obtain a mixed solution of γ-PGA / KMG;

[0044] (4) Divide the above mixed solution into 5ml centrifuge tubes, 4ml in each tube, then freeze at -20°C for 14h, then thaw at 13°C for 5h, cycle 3 times, put the formed complex solution in Dry in a vacuum dryer at 40°C for 20 hours to obtain composite fibers.

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Abstract

The invention belongs to the technical field of dietary fiber preparation and application, and particularly relates to a preparation method of a functional food for balancing intestinal flora and intestinal immunity. The method comprises the following steps: weighing poly gamma-glutamic acid, and adding purified water to prepare a poly gamma-glutamic acid solution; weighing glucomannan, and addingpurified water to prepare a glucomannan solution; dropwise adding the poly gamma-glutamic acid solution into the glucomannan solution, and centrifuging to remove bubbles to obtain a gamma-PGA/KMG mixed solution; and subpackaging the mixed solution into centrifugal tubes, carrying out freeze-thaw circulation, and drying to obtain the composite fiber. Under the synergistic effect of the glucomannanand the poly gamma-glutamic acid, the intestinal flora structure can be improved, and the intestinal function can be adjusted; the dietary fiber prepared by the preparation method has an efficient antibacterial function so that intestinal flora and intestinal immunity can be balanced, the intestinal flora can be balanced, and the activity of intestinal immune globulin A, mucoprotein and colon alkaline phosphatase can be increased.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of dietary fiber, and in particular relates to a preparation method of a functional food that balances intestinal flora and intestinal immunity. Background technique [0002] Poly-γ-glutamic acid [Poly-γ-glutamic acid, (γ-PGA)] is a typical polyelectrolyte, which is polymerized by D-glutamic acid and L-glutamic acid through γ-glutamine bonds The relative molecular weight of amino polymers is generally between 100,000 and 1 million. Compared with other polymeric polymer compounds, γ-PGA can be degraded into glutamic acid monomer in the body, which is necessary for the human body, has excellent biocompatibility, low immunogenicity, and no toxic side effects, which is unmatched by other materials of. [0003] As a proliferation factor of bifidobacteria, glucomannan can effectively promote the natural proliferation of bifidobacteria and other beneficial bacteria in the body, impr...

Claims

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Application Information

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IPC IPC(8): A23L33/175A23L33/125C12P13/02C12N1/20C12R1/10
CPCA23L33/175A23L33/125C12P13/02C12N1/20A23V2002/00A23V2200/3204A23V2200/324A23V2200/32A23V2200/308A23V2200/30A23V2250/0618Y02A50/30
Inventor 杨革孙阳车程川刘金锋巩志金
Owner QUFU NORMAL UNIV
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