Method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil

A technology of antibiotic resistance and secondary salinization, applied in the fields of soil preparation methods, agricultural machinery and implements, etc., can solve the problems of high cost, low efficiency, and reduction of soil antibiotic resistance genes, so as to reduce investment and improve Vegetable yield and quality, effect of improving microflora

Pending Publication Date: 2021-03-09
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is soil salinization control or soil antibiotic resistance gene reduction, the above-mentioned technologies can only achieve the purpose of repairing degraded soil unilaterally, and the cost is high and the efficiency is low.

Method used

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  • Method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil
  • Method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil
  • Method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: tomato field test

[0027] It is carried out in a facility vegetable cultivation base in Xianyang City, Shaanxi Province. The base has been established for 15 years, and organic fertilizers and chemical fertilizers are continuously applied all year round. The amount of application varies with different crops. Due to the serious problem of soil salinization due to excessive application of chemical fertilizers, the survey found that more than 80% of the 75 greenhouses had salinization problems. Four tomato greenhouses with serious salinization were selected for the experiment (the area is 55×9=495m2 2 ) were carried out, and two treatments were set up in each greenhouse, which were the control group and the treatment group respectively, and the control area was 45m 2 (5×9m 2 ), the area of ​​the treatment group is 450m 2 (50×9m 2 ). The test was carried out from August to September. The organic material used was fresh corn stalks, with an amount of 12 t...

Embodiment 2

[0029] Embodiment 2: laboratory simulation test

[0030] The soil tested was collected from a greenhouse vegetable greenhouse of an agricultural cooperative in Xianyang City, Shaanxi Province. The greenhouse has been continuously applied with large amounts of farmyard manure and chemical fertilizers for more than 10 years, and a large amount of base ions (nitrate nitrogen, potassium ions, etc.) and antibiotic resistance genes have accumulated in the soil. The simulation test was carried out in a laboratory incubator (at a temperature of 40° C.), setting a control group and a treatment group, and repeating 3 times. The control group did not do any treatment, while the treatment group firstly mixed the soil with crushed (about 2cm) wheat straw (amount of 6g kg -1 dry soil) and put it into a wide-mouth plastic bottle, pour water until 5cm of water accumulates on the soil surface, cover the soil surface with a 6-thread plastic film to seal, and then put all the culture units into...

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Abstract

The invention discloses a method for synchronously reducing secondary salinization and antibiotic resistance genes of greenhouse soil. The method comprises the following steps of picking up plant residues and plastic film residues after harvesting of previous crop of vegetables, and moving the picked-up plant residues and plastic film residues out of a greenhouse; uniformly spreading fresh organicmaterials into the soil surface, turning and burying the organic materials by using a root rotating machine, and uniformly mixing the organic materials with the surface soil; performing saturated irrigation on the soil, and finally quickly covering a plastic film on the ground surface, so that the organic materials are subjected to anaerobic degradation under the strong reduction condition created by irrigation and film covering; and after a plurality of days of strong reduction treatment, removing the plastic film, and draining surface accumulated water to obtain the greenhouse facility soilwith significantly reduced antibiotic resistance gene abundance and significantly relieved secondary salinization. The method can rapidly and synchronously reduce antibiotic resistance genes of the greenhouse soil and relieve soil secondary salinization, and has an improvement effect on degraded facility vegetable soil; and in addition, soil organic matter can be increased, soil denitrification is stimulated, accumulation of nitrate nitrogen in the soil is reduced, and soil fertility is improved.

Description

technical field [0001] The invention relates to the technical field of soil treatment, in particular to a method for simultaneously reducing greenhouse soil secondary salinization and antibiotic resistance genes. Background technique [0002] As the world's largest producer of vegetables, my country has a planting area of ​​over 20 million hectares and an annual output of 700 million tons. Greenhouse vegetables, as a high-efficiency vegetable cultivation mode, break the restrictions of season and climate in conventional cultivation, and play an important role in ensuring people's demand for vegetables (especially off-season vegetables). In addition, the advantages of high input, high output, high yield, and intensification of vegetables in greenhouses have also greatly encouraged farmers' enthusiasm for planting and increased farmers' income. According to the survey, in 2016, the planting area of ​​greenhouse vegetables in my country exceeded 3.9 million hectares, the annua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01B79/02
CPCA01B79/02
Inventor 陈艳龙张雨涵王宇恒米卉子陈帆叶訚
Owner NORTHWESTERN POLYTECHNICAL UNIV
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