A method for hybrid breeding of low-oxygen tolerant blue grouper

A blue grouper, hybrid breeding technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of slow growth and low breeding efficiency of blue grouper, and achieve considerable economic benefits, high market acceptance, and significant The effect of growth advantage

Active Publication Date: 2021-12-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for hybrid breeding of low-oxygen-resistant blue grouper, aiming to solve the problem that the blue grouper in the prior art grows slowly and causes its breeding benefit to be low

Method used

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  • A method for hybrid breeding of low-oxygen tolerant blue grouper
  • A method for hybrid breeding of low-oxygen tolerant blue grouper
  • A method for hybrid breeding of low-oxygen tolerant blue grouper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment one blue body large grouper and blue grouper are cultivated

[0061] The breeding environment of blue-bodied grouper is factory farming. The size of the breeding pool is 6m×6m×3m. There are 30 blue-body grouper in each pond, and a total of 80 blue-body grouper. 50-70cm, weight 20-30Kg. The dissolved oxygen (DO) in the pool is 7-10 mg / L, and the water exchange volume is 3-4 m 3 / h, using natural light. The water temperature is 23-24°C in October of the first year, and gradually drops to 18-20°C in December, and then gradually rises to 25-26°C in March of the next year; When the water temperature is stable at 25-26°C, mature male fish can be selected to collect semen. Wherein, the bait fed to the parents is any one of mackerel and Spanish mackerel or mixed in any proportion of the two, and the feeding amount is about 2-3% of the body weight of the parent fish. Pour the pool regularly every day to keep the water fresh.

[0062] The blue grouper is cultured ...

Embodiment 2

[0063] Example 2 Cryopreservation of the blue-bodied grouper sperm

[0064] (1) Acquisition of mature sperm

[0065] Under the artificially controlled environment, the sperm production time of the blue-bodied grouper extends from April to mid-September. At this time, the precious and small amount of blue-bodied grouper sperm can be preserved for a long time by freezing sperm technology, which can be used Insemination with blue grouper eggs in the following year.

[0066] The semen is collected every half month during the reproductive season, and the male fish that have collected the semen are put into another fish pond with fresh water quality. Before semen collection, MS-222 was used to anesthetize the whole pool. The male fish was placed on the delivery bed, and the abdomen was squeezed by hand. Mature males with semen flow were selected, and the semen was obtained by squeezing the abdomen. During the process of semen extraction, sea water should be avoided, otherwise the ...

Embodiment 3

[0075] Example 3 Artificial insemination of blue-bodied large grouper frozen sperm and blue grouper eggs

[0076] (1) blue grouper induced labor

[0077] When the water temperature reaches 23-26°C in April, select a well-developed female blue grouper with a swollen abdomen; inject chorionic gonadotropin and gonadotropin-releasing hormone analogs into the base of the pectoral fins, and the dose of chorionic gonadotropin is 200 -300IU / kg, the dose of gonadotropin-releasing hormone analogue is 35μg / kg, the two are mixed in 0.9% normal saline, and each fish is injected with about 1mL. After 48-60 hours of injection, suck out the eggs through a straw to observe the development status, select mature female fish, place them on the spawning bed, squeeze the abdomen of the fish to collect eggs, and then collect the eggs in a dry plastic basin, and obtain a total of 10Kg of eggs .

[0078] (2) Fertilization of eggs with frozen sperm

[0079] During the 48-60 hours after the blue grou...

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Abstract

The invention discloses a method for hybrid breeding of low-oxygen tolerant blue grouper. The invention comprises the following steps: 1) cultivating the parent fish of the blue grouper and obtaining the sperm; 2) cryopreserving the sperm; 3) cultivating the parent fish of the blue grouper and obtaining the eggs; 4) artificial insemination to obtain the parent fish of the blue grouper Grouper frozen semen, thawed, mixed with eggs, added to seawater for fertilization; left to stand, added to high-salinity seawater, and collected floating eggs; Eggs are placed in a breeding pond for hatching and cultivating; 6) later stage cultivation, using biological bait for cultivation to obtain blue grouper hybrid fry. The blue grouper hybrid breeding of the present invention has high seedling survival rate, fast growth rate, low oxygen resistance, low temperature resistance, uniform shape and body color of hybrid offspring, chromosomal karyotype is 2n=1sm+47t, has unique genetic characteristics and Significant hybrid advantage, high market acceptance, considerable economic benefits.

Description

technical field [0001] The invention belongs to the technical field of fish cross-breeding, in particular to a method for cross-breeding hypoxia-tolerant blue grouper. Background technique [0002] Grouper is the main marine farmed and captured fish in the world. In 2019, the production of grouper aquaculture and fishing in my country reached 183,127 tons and 97,411 tons respectively, ranking second in marine cultured fish, with an output value of more than 20 billion yuan. [0003] blue grouper ( Epinephelus awoara ) taxonomically belongs to Perciformes (Perciformes), grouper family (Epinephelidae), grouper ( Epinephelus ), commonly known as soil spots. Blue grouper is a rare and rare fish inhabiting the middle and lower layers of warm water. It is often distributed in the western part of the North Pacific Ocean. In my country, it is mostly distributed in the South China Sea and the southern part of the East China Sea. [0004] Green grouper has high nutritional value,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 田永胜陈帅王林娜李振通李子奇黎琳琳刘阳
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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