Composition for biologically preventing and treating cotton blight and verticillium wilt as well as preparation method and application thereof
A technology of verticillium wilt and a composition is applied in the field of biological control of cotton verticillium wilt and its preparation field, which can solve the problems of destruction of beneficial bacteria, unsatisfactory pollution-free cotton verticillium wilt, etc. The effect of wilt occurrence and low cost
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[0038] The preparation method reference example 1 of tobacco leaf extract
[0039] Crush 1 part by weight of dried tobacco leaves to pass through a 10-mesh sieve, add 12 parts by weight of water, adjust the pH value to 11, heat and leach at 50°C for 1.5 hours, filter, and concentrate the filtrate to a moisture content of 25%. Tobacco leaf extract 1 was obtained.
[0040] The preparation method reference example 2 of tobacco leaf extract
[0041] Crush 1 part by weight of dried tobacco leaves to pass through a 10-mesh sieve, add 10 parts by weight of water, adjust the pH value to 12, heat and leach for 2.0 hours at 40°C, filter, and concentrate the filtrate to a moisture content of 20%. Tobacco leaf extract 2 was obtained.
[0042] The preparation method reference example 3 of tobacco leaf extract
[0043] After crushing 1 part by weight of dried tobacco leaves to pass through a 10-mesh sieve, add 15 parts by weight of water, adjust the pH value to 10, heat and leach for 1.0...
Embodiment 1~3
[0051] The components and weight parts of Examples 1 to 3 of a composition for biological control of cotton Fusarium wilt are shown in Table 1.
[0052] Table 1 Components and parts by weight of Examples 1 to 3 of a composition for biological control of cotton Fusarium wilt
[0053]
[0054] Note: "-" in the table means not added.
Embodiment 1
[0056] According to the components and parts by weight of Example 1 in Table 1, the Bacillus firmus preparation, Paecilomyces lilacinus preparation, Streptomyces scabies preparation, γ-aminobutyric acid and organic materials were first carried out once at a speed of 40r / min. After stirring and mixing for 25 minutes, ferment for 60 hours at 56°C with a water content of 55%, then add tobacco leaf extract and spinach extract, and carry out secondary stirring and mixing for 15 minutes at a speed of 40r / min to obtain biological control of cotton fusarium wilt Composition 1.
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