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A plant Bacillus gbw-f008 producing indole acetic acid and its application

A technology of GBW-F008 and Bacillus, applied in the field of microorganisms, can solve problems such as soil and plant hazards, achieve the effects of increasing average fruit weight, strong in-situ growth-promoting effect, and increasing content

Active Publication Date: 2022-03-01
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there are very few fungi that integrate the above-mentioned functions and can be used in soil microbial technology, which has led to the use of hormones such as naphthaleneacetic acid and nutrients such as trehalose in many microbial products instead of microorganisms for plants. Planting, although it will also have a growth-promoting effect on plants, it will also cause certain harm and negative effects on soil and plants

Method used

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  • A plant Bacillus gbw-f008 producing indole acetic acid and its application
  • A plant Bacillus gbw-f008 producing indole acetic acid and its application
  • A plant Bacillus gbw-f008 producing indole acetic acid and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Isolation, screening and identification of Bacillus GBW-F008 in plants

[0079] 1. Isolation and screening of Bacillus GBW-F008 in plants

[0080] Roots of crops were taken from perennial vegetable greenhouses in Hebei Langfang, Shandong Jinan, Tai'an, Laiyang, Zibo, Liaocheng, and Shouguang, put them in medical test sample bags, stored in a refrigerator at 4°C, and separated in time. The samples involved loofah, muskmelon, and pointed The roots of 56 kinds of crops such as peppers, colorful peppers, tomatoes, cucumbers, etc. Take 2g of the sample, put it into 18mL sterile water, shake and extract at 30°C and 200rpm / min for 30min, put the sample solution in a water bath at 75°C for 20min, and then carry out gradient dilution. Take 100μl of each gradient dilution solution and spread it on the NA plate. The coated plate was cultured upside down at 37°C for 48 hours, single colonies of different shapes were picked out with sterilized bamboo sticks, transferred ...

Embodiment 2

[0087] Example 2: Growth and physiological and biochemical characteristics of Bacillus GBW-F008 in plants

[0088] 1. Physiological and biochemical characteristics of GBW-F008

[0089] After culturing Bacillus intraplanta GBW-F008 in the improved NB medium, according to the physiological and biochemical detection methods in the "Bergery Bacterial Identification Manual" and "Common Bacterial System Identification Manual", the physiological and biochemical characteristics of the bacteria were measured, and the results were as follows: Table 1 shows. Bacillus intraplanta GBW-F008 can grow and reproduce in the temperature range of 20°C to 42°C, but its most suitable growth temperature is 37°C, and it can grow normally in the modified NB medium with pH 4.5 to 9.6, and the optimum growth pH The range is 6.5-7.5, the bacteria can tolerate up to 10% NaCl, can produce urease, casein, catalase and oxidase reaction are positive, can use citrate; GBW-F008 can also use xylose, inulin , g...

Embodiment 3

[0094] Embodiment 3: Comparison of the ability of Bacillus GBW-F008 to produce indoleacetic acid under different conditions

[0095] 1. Comparison of quantitative determination methods for IAA produced by GBW-F008

[0096] The standard product of indole acetic acid IAA was purchased from Sigma, with a molecular weight of 175.18 g / moL and a chemical formula of C 10 h 9 NO 2 .

[0097] Using absolute ethanol as a solvent, prepare standard products with IAA concentrations of 10, 20, 30, 40, and 50 mg / L, take 100 μl of IAA standard solution, and add 100 μl of Salkowski colorimetric solution in equal volume. A microplate reader was used to measure the OD540nm absorbance values ​​after 0 min and 30 min of dark reaction respectively. Take the OD difference between the two as the abscissa and the IAA concentration as the ordinate to draw a colorimetric standard curve ( Figure 4 ), the standard curve has good linearity, R 2 = 0.9999.

[0098] Using methanol as a solvent, prepar...

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Abstract

The invention discloses an indole acetic acid-producing bacillus GBW‑F008 and its application. The classification of the Bacillus endophyticus GBW-F008 is named Bacillus endophyticus, and the preservation number is CGMCC No.20919. Its colony is white, opaque, round, with neat edges, moist and shiny surface, and a diameter of 1-5mm; The bacterium is cylindrical, with a diameter of 2-3μm; the proximal end of the spore is oblong, with flagella around it, and the growth temperature range is wide. The Bacillus intraplanta GBW‑F008 has the ability to produce various enzymes and indoleacetic acid, and can promote the release of available phosphorus and available potassium in tomato planting soil, increase the content of free amino acids and indoleacetic acid in the soil, and significantly improve the tomato production capacity. Stem thickness, strong seedling index, increase the average fruit weight of tomato, thereby improving the yield and quality of tomato, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an indole acetic acid-producing bacillus GBW-F008 and its application. Background technique [0002] Tomatoes are very popular because of their nutritious fruit, sweet and sour taste, juicy juice and various eating methods. In the process of tomato planting, tomato fruits are often too small, the root system is underdeveloped, and the fruit setting rate is low. To solve these problems, fruit farmers often choose to use hormones or pesticides to increase nutrients and promote plant growth. However, hormones or pesticides Not only will it affect the taste of tomatoes, but it will also cause drug resistance in tomato plants and cause soil pollution. [0003] Rhizosphere bacteria are a type of microorganisms that can use the nutrients in the plant rhizosphere, including root exudates and lysates, to grow and reproduce. They can also synthesize and secrete some met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/10C05F11/08C05G3/00C05G3/80C09K17/14A01N63/22A01N43/38A01P21/00C12R1/07
CPCC12P17/10C05D1/00C05F11/08C05G3/00C05G3/80C09K17/14A01N63/22A01N43/38C05F9/04C05F11/02
Inventor 李慧芬王金龙魏秉培张大伟樊连梅解西柱陈四明孟德坤
Owner QINGDAO SHANGDE BIOTECH
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