Magnetic 3D gel guide pipe for repairing nerve defects and preparation method and application thereof

Abstract

The invention discloses a magnetic 3D gel guide pipe for repairing nerve defects and a preparation method and application thereof. The magnetic 3D gel guide pipe is prepared from the following components in parts by weight: 97-99 parts of an alginic acid / chitosan mixture, 0.8-1.5 parts of Fe3O4 and 0.8-1.5 parts of GO, the magnetic 3D gel guide pipe is of a spiral structure, the screw pitch is 30[mu]m-60[mu]m, the inner diameter of the guide pipe is 1mm-8mm, and the outer diameter of the guide pipe is 2mm-10mm. The preparation method of the magnetic 3D gel guide pipe comprises the following steps that a spinning solution is prepared, specifically, the alginic acid / chitosan mixed solution, a Fe3O4 dispersion solution and a GO dispersion solution are mixed, and normal-temperature ultrasonictreatment is carried out to obtain a spinning solution; and the magnetic 3D gel guide pipe is prepared, the spinning solution is pumped into a micro-fluidic chip from a main liquid phase inflow channel, a calcium chloride solution and absolute ethyl alcohol are pumped into the micro-fluidic chip from different auxiliary liquid phase channels, the pumping speeds of the spinning solution, the calcium chloride and the absolute ethyl alcohol is adjusted, and the magnetic 3D gel guide pipe is prepared. According to the magnetic 3D gel guide pipe for repairing the nerve defects and the preparation method and application thereof, a microfluidic technology is adopted, and the prepared guide pipe can safely and effectively promote rapid repair of peripheral nerve defects.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a magnetic 3D gel catheter for repairing nerve defects and its preparation method and application. Background technique [0002] After nerve damage occurs, the self-regeneration and repair ability is poor, especially for long-segment nerve defects. At present, the gold standard for clinical repair of long-segment nerve defects is autologous nerve transplantation. In addition, there are stump sutures and allogeneic transplantation. The repair effect is not ideal, and it is accompanied by many complications, such as: autologous nerve transplantation leads to nerve donor area Denervation dysfunction, suturing of stump ends causes excessive tension of nerve stumps, which in turn leads to secondary nerve necrosis, graft rejection, etc. Nerve tissue engineering is the product of the combination of nerve histology and materials engineering. It is an important branch of tissue engine...

AI Technical Summary

Problems solved by technology

However, the repair effect of the artificial "neural prosthesis" constructed so far is not ideal, as follows: ① It is difficult to achieve histological repair of large defects (≥3 cm); ② The function of the regenerated nerve is not good; Long time required for regeneration or repair; Difficulty in achieving directional extension of axons

Microfluidic spinning technology adopts the principle of coaxial laminar flow, which has been widely used in the preparation of industrial materials, but it is still in its infancy in the field of tissue engineering. At present, the 3D tubular gel prepared by microfluidic technology in the field of tissue engineering fewer catheters

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