Mutant gene OsABCC1 for regulating and controlling cadmium and arsenic accumulation of rice and application of mutant gene OsABCC1
A technology for mutating genes and rice, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of less research on the mechanism
Active Publication Date: 2021-03-16
CHINA NAT RICE RES INST
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Problems solved by technology
However, there are few studies on the mechanism of the ABCC gene family involved in the transport and accumulation of cadmium and arsenic in rice.
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[0015] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0016] The present invention uses japonica rice (Oryza_japonica) Nipponbare as wild-type seeds (M 0 Generation) and undergo EMS mutagenesis treatment, plant the treated seeds to obtain M 1 Substitute plants. m 1 Generation plants selfed to produce seeds (M 2 generation), planting M on the field 2 After husking the harvested rice grains, the content of cadmium and arsenic in brown rice was determined by inductively coupled plasma mass spectrometry (ICP-MS), and the plants with high accumulation of cadmium and arsenic were screened. The obtained rice lines with high accumulation of cadmium and arsenic were further screened by selfing, and planted in different paddy fields for verification. A single mutant homozygous single plant with high accumulation of cadmium and arsenic and stable inheritance was obtained, named as hca1 (highcadmium and arsenic ...
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The invention discloses a mutant gene OsABCC1 for regulating and controlling cadmium and arsenic accumulation of rice and application of the mutant gene OsABCC1. According to the mutant gene OsABCC1 for regulating and controlling cadmium and arsenic accumulation of rice, a first base behind a tenth exon of a wild type OsABCC1 gene is subjected to variable shear mutation, namely, a base at a 2261 nucleotide at the downstream of ATG is mutated into A from wild type G, so that 1116 to 1131 nucleotides in a corresponding coding nucleotide sequence are deleted, a mutant is deleted and changed at 372<nd> to 382<nd> amino acids and is translated at a 383<rd> amino acid to be terminated in advance, the coding nucleotide sequence is shown as SEQ ID No.1, and the cadmium and arsenic contents inrice grains of the mutant hca1 are higher than those in wild type rice grains. The amino acid sequence coded by the mutant gene OsABCC1 is shown as SEQ ID No. 2. According to the application of the mutant gene OsABCC1, the rice contains the mutant gene OsABCC1 or contains the coding amino acid of the mutant gene OsABCC1. The cadmium and arsenic contents in the grains of the obtained mutant material hca1 are respectively increased by about 1 time and 5 times.
Description
technical field [0001] The invention belongs to the field of crop genetics and breeding, and specifically relates to a mutant gene for regulating the accumulation of cadmium and arsenic in rice OsABCC1 and its application. Background technique [0002] Cadmium (Cd) and arsenic (Arsenic, As) are the primary pollutants that affect the soil environmental quality of agricultural land in my country, and have strong toxic effects on animals and plants. Rice is the most important food crop in my country. Compared with dry food crops such as wheat and corn, rice has a stronger ability to absorb and enrich cadmium and arsenic, which poses a potential health risk to people with high consumption of rice. The control of heavy metal pollution in farmland is conducive to reducing its phytoavailability, preventing the transfer of heavy metals to rice grains, and ensuring normal agricultural production and rice quality and safety. Among them, phytoremediation technology is an emerging gre...
Claims
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Login to View More IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8243
Inventor 陈铭学杨欢牟仁祥曹赵云朱智伟
Owner CHINA NAT RICE RES INST