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Plate

A flat plate and substrate technology, applied in biochemical instruments, using thermal variables to measure fluid velocity, applying thermal effects to detect fluid flow, etc., can solve problems such as cumbersome operations and difficulties in determining the situation of the micro-flow path being observed

Pending Publication Date: 2021-03-19
USHIO DENKI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Such work is extremely cumbersome, especially when tens to hundreds of tiny microchannels are arranged at a high density, even if the magnification of the microscope is reduced, it is difficult to identify the microchannel being observed.

Method used

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Effect test

no. 1 approach 〉

[0042] figure 1 It is a plan view showing the structure of the tablet concerning 1st Embodiment of this invention. figure 2 Yes figure 1 A-A sectional view of the plate shown. image 3 Yes figure 1 B-B sectional view of the plate shown.

[0043] This flat panel 10 is constituted by a plate-shaped body in which transparent first substrate 11 and second substrate 15 are stacked and integrally bonded. The plate 10 of this example has a plurality of (three in the illustrated example) linear microchannels 16 inside. These microchannels 16 are formed independently without communicating with each other, and are arranged in parallel.

[0044] On the first substrate 11, on the surface on the side contacting the second substrate 15 (in figure 2 (middle is the lower surface) is formed with a channel forming groove 12 . On the other hand, the surface of the second substrate 15 on the side contacting the first substrate 11 (in figure 2 middle is the upper surface) becomes a f...

no. 2 approach 〉

[0066] Figure 4 It is a plan view showing the structure of the tablet concerning 2nd Embodiment of this invention. This flat plate 10 is the same as the flat plate 10 related to the first embodiment, and consists of a transparent first substrate 11 and a second substrate 15 (refer to figure 2 ) are stacked and integrally bonded to form a plate-shaped body. Inside the plate 10, a plurality (three in the illustrated example) of linear microchannels 16 are independently formed without communicating with each other, and are arranged in parallel.

[0067] In a plan view of the first substrate 11 , a weld line by injection molding is formed on the first substrate 11 along the opening edge of the injection port 13 and the discharge port 14 to the periphery of the first substrate 11 . Here, since the injection port 13 and the discharge port 14 penetrating in the thickness direction are formed on the first substrate 11, when the first substrate 11 is produced by injection molding a...

no. 3 approach 〉

[0071] Figure 5 It is a plan view showing the structure of the tablet concerning 3rd Embodiment of this invention. This flat plate 10 is the same as the flat plate 10 related to the first embodiment, and consists of a transparent first substrate 11 and a second substrate 15 (refer to figure 2 ) are stacked and integrally bonded to form a plate-shaped body. Microchannels 16 are formed inside the plate 10 .

[0072] In this plate 10 , the microchannel 16 has a source channel 17 communicating with the injection port 13 and a plurality of (three in the illustrated example) branch channels 18 communicating with the source channel 17 . The branch flow paths 18 are respectively communicated with the discharge ports 14 formed corresponding to the branch flow paths 18 .

[0073] In addition, in the plate 10 of this example, identification marks 20 formed of one-dimensional codes or two-dimensional codes are formed in the vicinity of the source channel 17 and the branch channel 18 ...

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Abstract

Provided is a plate having a plurality of micro flow paths or a micro flow path that has a plurality of branch flow paths formed therein, wherein when a sample flowing through the micro flow paths isobserved using a microscope, the position of the micro flow path or branch flow path being observed can be easily specified without reducing the magnification of the microscope. The plate has the micro flow paths therein and is characterized by having identification marks for identifying the positions of the micro flow paths in the surface direction of the plate. When the plate has the plurality of micro flow paths formed independent of each other, it is preferable for the identification marks to be respectively formed for each of the micro flow paths. When the micro flow path has a source flow path communicating with an injection port to which a sample is injected and a plurality of branch flow paths communicating with the source flow path, it is preferable for the identification marks tobe respectively formed for the source flow path and each of the branch flow paths.

Description

technical field [0001] The present invention relates to a plate having a microchannel therein, and more specifically, to a plate suitable as a culture vessel capable of culturing and observing cells and living tissue within the microchannel. Background technique [0002] Cells carry out their functional control in the extracellular microenvironment of the organism. Here, the extracellular microenvironment is composed of growth factors, soluble factors such as vitamins and gas molecules, insoluble factors such as extracellular matrix, and intercellular interactions. [0003] Conventionally, for culturing cells and living tissues, culture vessels such as petri dishes and culture plates formed with culture media such as agar have been used. Cell culture using such a culture container is carried out in a two-dimensional (flat) environment, so it is difficult to reproduce the extracellular microenvironment. Under such circumstances, a flat plate (biochip, microchip) having a mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B81B1/00C12M1/00G01N37/00
CPCC12M25/16C12M41/36B01L3/5027B01L2300/0864B01L2300/021B01L2300/0887B81B1/006B81B2201/05B81B2203/0338B81C99/007C12M23/12C12M23/16C12M23/50
Inventor 野元大辅山中诚
Owner USHIO DENKI KK
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