Small insect fixing device for microinjection

Abstract

The invention discloses a small insect fixing device for microinjection. For the problems that existing various anesthetic drugs can cause poor physical states of insects, and even death, and the result of an injection experiment is seriously affected, the scheme is provided as follows: the small insect fixing device comprises a bottom plate; a mounting pipe is fixedly connected with the center position of the outer wall of the top part of the bottom plate; the inner wall of the bottom part of the mounting pipe is connected with a second threaded rod through a bearing; the outer wall of the second threaded rod is in threaded connection with a threaded sleeve; the top end of the threaded sleeve is fixedly connected with a mounting plate; and one side of the outer wall of the top part of themounting plate is connected with a fixing frame through a hinge. According to the small insect fixing device, airbag cushions, made of a relatively soft material, at the bottom parts of movable rodscorrespondingly are used, and since the bottom part of the mounting plate is provided with a magnetic ring, the fixing frame can be attracted to the mounting plate under the natural state, and is effectively fixed by a physical method, thus avoiding a series of negative effects caused by chemical anesthetics.

Description

technical field [0001] The invention relates to the technical field of microinjection, in particular to a small insect fixing device for microinjection. Background technique [0002] At present, under a high-power inverted microscope, a micromanipulator is used to control the mechanical device of the microinjection needle to move within the field of view of the microscope, which is a method for operating cells or early embryos. There is no limit on the length of exogenous gene transfer by microinjection. It has been proved that DNA fragments of hundreds of kb can be successfully produced into transgenic animals. The disadvantages are that the equipment is sophisticated and expensive, the operation technique requires long-term practice, and only a limited number of cells can be injected each time. The micropipettes used in these operations are made with a capillary puller, which heats the glass capillary to its melting temperature and then draws it to the appropriate diamete...

AI Technical Summary

Problems solved by technology

[0003] The existing small-sized insect fixing devices for microinjection have the following problems: before the existing insect microinjection, most of the insects need to be anesthetized to a certain degree, but the existing many types of anesthetic drugs will cause the body of the insect to State deterioration, even death, seriously affects the results of injection experiments, and existing patents are not easy to solve such problems, therefore, there is an urgent need for a small insect fixation device for microinjection to solve the above problems

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