Unlock instant, AI-driven research and patent intelligence for your innovation.

Use of compound for promoting cholesterol metabolism

A cholesterol, use technology, applied in the field of new use of the compound, can solve problems such as cholesterol deposition, coronary artery disease, atherosclerosis, etc.

Inactive Publication Date: 2021-03-26
百岳特生物技术(上海)有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, a defect in the LDL receptor reduces the uptake of LDL by cells, allowing LDL to accumulate in the blood, leading to the deposition of cholesterol in the arterial walls, and ultimately atherosclerosis and coronary artery disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of compound for promoting cholesterol metabolism
  • Use of compound for promoting cholesterol metabolism
  • Use of compound for promoting cholesterol metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Preparation of compounds with cholesterol-lowering activity

[0057] In order to obtain the compound with cholesterol-lowering activity, a tartary buckwheat seed coat extract was prepared first. Briefly, the hull of the whole grain of tartary buckwheat, that is, the seed coat of tartary buckwheat, is first washed and dried, and pulverized using a grinder. Secondly, the pulverized tartary buckwheat seed coat is extracted with water as a solvent. The weight ratio of the solvent to the pulverized tartary buckwheat seed coat is in the range of 15:1 to 10:1. The extraction temperature is between 70°C and 90°C. The extraction time in this embodiment is 1 to 2 hours.

[0058] In order to remove residual solids, after the tartary buckwheat seed coat extract obtained in the above extraction step is cooled to room temperature, it can be further filtered through a 400-mesh filter to obtain a supernatant. The supernatant can be further concentrated under reduced pressure at 40°...

Embodiment 2

[0069] Validation of compound cholesterol-lowering activity

[0070] In order to verify the effect of compounds FT-01, FT-02, and FT-03 on regulating the expression of genes related to cholesterol metabolism, human liver cancer cell line HepG2 was given 2 mL of cell culture medium containing 50 μg / mL of any one of the three compounds, or Cells were treated with cell culture medium only (control group). Cells in each group were cultured at 37°C for 24 hours and then used for gene expression analysis. The target genes included SCARB1, APOA1, and LDLR genes.

[0071] Figure 8 Show the relative expression of each target gene in the aforementioned groups of HepG2 cells; *** and *** Respectively represent p Figure 8 , compared with the control group, the administration of FT-01 (ie rutin) could not improve the expression of SCARB1, APOA1, and LDLR genes. In contrast, administration of FT-02 (ie, larisinolate 4'-O-β-D-glucopyranoside) significantly increased the expression of SC...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of new application of compounds, in particular to use of a compound for promoting cholesterol metabolism. The present invention provides use of a composition for preparing a pharmaceutical product for promoting cholesterol metabolism-related gene expression, wherein the composition comprises a pharmaceutically acceptable carrier, and a compound selected from thegroup consisting of larisinol 4'-O-[beta]-Dpyranoside, adenosine, and combinations thereof. The cholesterol metabolism related gene comprises SCARB1, APOA1 and LDLR. By promoting the expression of thegenes, the larisinol 4'-O-[beta]-Dpyranoside and adenosine can reduce the risk of suffering from cardiovascular diseases due to poor cholesterol metabolism in individuals.

Description

technical field [0001] The present invention relates to a new field of application of a compound, in particular to the application of a compound that promotes cholesterol metabolism. Background technique [0002] Cholesterol metabolism in the human body involves multiple organs, the most important of which is the liver. According to previous studies, cellular cholesterol content is regulated by a complex balance involving endogenous synthesis of cholesterol, cellular uptake of cholesterol, and excretion of intracellular cholesterol from the cell to the blood. [0003] Cholesterol is transported in the blood in the form of cholesterolipids packaged in vesicles with other lipids and proteins. The intestinal tract assembles dietary cholesterol into chylomicrons, which are transported through the blood and finally absorbed by the liver, which is also the main site of cholesterol synthesis in the human body. The liver packages dietary and newly synthesized cholesterol into low-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7048A61K31/7076A61P3/10A61P3/00
CPCA61K31/7048A61K31/352A61K36/70A61P1/16A61P39/06A61K31/708A61K2236/331A61K31/7076A61P3/00A61P3/10A61K2300/00A61K31/52
Inventor 林咏翔
Owner 百岳特生物技术(上海)有限公司