Cow dung bio-organic fertilizer as well as preparation method and application thereof
A bio-organic fertilizer and a technology of organic fertilizer, applied in the field of agricultural soil fertilizer, can solve the problems such as good growth-promoting effect, poor effect, difficult survival, etc., so as to improve income and crop quality, promote banana growth, reduce The effect of the occurrence of pests
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Embodiment 1
[0020] Preparation of bacterial solution
[0021] Taking the rhizosphere soil of the banana garden as the research object, Bacillus jelly jelly and Rosemonospora obtained after isolation and purification were screened. After activation, one ring of activated colonies was picked respectively, and inoculated into 1L LB culture medium at 30°C. In a constant temperature shaking incubator, culture at 130 rpm for 24 hours to form a bacterial liquid.
[0022] Prepare several Erlenmeyer flasks with a capacity of 500mL, pour 200mL of PDA (pH value adjusted to 7.0) culture solution into the 500mL Erlenmeyer flasks, and carry out high-temperature sterilization after sub-packaging, and set aside.
[0023] The two cultured bacterial solutions were inoculated into a 500mL Erlenmeyer flask containing 200mL of PDA culture solution according to the volume ratio of the bacterial solution and the culture solution at a ratio of 1:100, and then cultivated at 33°C for 48h at a shaker speed of 100rp...
Embodiment 2
[0025] 1. Preparation of bio-organic fertilizer 1
[0026] (1) Raw material preparation: first mix the raw material molasses fermentation liquid and pumice, and then mix with the raw material cow dung, traditional Chinese medicine slag, crushed fruit peel, potassium ore powder, and phosphate rock powder;
[0027] The mass ratio of dry matter mixed with cow dung, traditional Chinese medicine residue, peel crushed material, potassium ore powder, phosphate rock powder, molasses fermentation liquid and pumice is 55:20:30:20:6:7.5:15;
[0028] The traditional Chinese medicine dregs are composed of herbaceous root dregs, pyrethrum dregs, eucalyptus dregs and Luoxing herbal dregs; the composition dry matter mass ratio is 1:1~1.3:1.5~2:0.8~1.0;
[0029] Pumice is granular solid, 0.01cm<particle size≤0.5cm;
[0030] The solid matter content of the molasses fermented liquid is 50%-60%.
[0031] (2) Primary fermentation: After the above-mentioned materials are mixed evenly, add 1% to 3...
Embodiment 3
[0074] field test
[0075] 1. Materials and methods
[0076] The tested banana variety was Brazilian banana.
[0077] Bio-organic fertilizers for testing: sample bio-organic fertilizer 1, bio-organic fertilizer 2, bio-organic fertilizer 3, bio-organic fertilizer 4, bio-organic fertilizer 5, organic fertilizer 6 and conventional fertilizer for testing.
[0078] Test site: Lvxin Banana Planting Base, Luoxu Town, Wuming District, Nanning City. Seven treatments were set up in the test area, with 10 rows for each treatment and 20 plants in each row. A total of 200 plants were planted with a row spacing of 2m×2.5m. Fertilization was applied for the first time on March 27, 2017 in the test area, and for the second time on August 2.
[0079] 2. Experimental design
[0080] The experimental design consists of 7 treatments, namely:
[0081] Treatment 1: sample bio-organic fertilizer 1 (fertilization twice), hereinafter referred to as F1.
[0082] Treatment 2: sample bio-organic fer...
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