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A kind of cell sorting method and chip
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A sorting and cell technology, applied in the field of biomedicine, can solve the problems of precise separation of cells that cannot be approached in size, long chip length, and inability to release cells on demand
Active Publication Date: 2022-05-24
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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However, its sorting performance is affected by the cross-sectional shape of the micro-column. As the cross-sectional shape of the micro-column is mostly used, the circular shape has a low ability to control the flow rate in the micro-column gap, and there are low sorting flux, easy clogging, and required cycle time. Disadvantages of many and long chip length
At the same time, it is impossible to accurately separate cells with similar sizes using traditional micro-pillar cross-sectional shapes such as circles, such as larger white blood cells and smaller circulating tumor cells.
[0005] The traditional size-based cell capture technology is to set an array of microgrooves in the middle of the flow channel to capture cells, and rely on the reverse flow of fluid to release the captured cells. Reverse flow to release all trapped cells, cannot release cells on demand
It still cannot meet the needs of rare cells for high separation throughput, low clogging probability, high recovery rate, and high purity sorting
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[0085] see Figure 5 , Image 6 , Figure 7 As shown, the chip includes a sample inlet 1, a buffer inlet 2, a primary sorting microcolumn 3, a transition channel 6, a buffer inlet 7, a capture and release area 8, an array capture and release area 9, an outlet 10, and a bottom outlet 11. , the primary sorting area 13 , the capture area 14 , the storage area 15 , the release channel 16 , the interception channel 17 , and the deformation channel 18 .
[0086] In this example, the size of the target cells is larger than that of all background cells, and the sample inlet 1 is located on the bottom side. As the liquid flows, the target cells gradually move away from the bottom side and approach the top side, so the transition channel 6 is connected to the primary sorting area. 13 on the top side. The buffer fl...
Embodiment 2
[0104] Embodiments of the present invention provide a cascade array microstructure multifunctional cell sorting and capturing microfluidic chip and a method of using the same.
[0105] see Figure 8 , Figure 9 As shown, the chip includes sample inlet 1, buffer inlet 2, primary sorting microcolumn 3, secondary sorting microcolumn 4, top outlet 5, transition flow channel 6, buffer inlet 7, capture and release area 8, array Capture release area 9 , outlet 10 , bottom outlet 11 , secondary sorting area 12 , primary sorting area 13 , capture area 14 , storage area 15 , release channel 16 , retention channel 17 , deformation channel 18 .
[0106] In this example, the target cells are large in size, with small-sized background cells and large-sized background cells co-existing, and the deformability of the target cells is greater than that of large-sized background cells, and the sample inlet 1 is located on the bottom side. During the flow, the target cells and the large-sized ba...
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Abstract
The present invention relates to the field of biomedicine, and in particular provides a cell sorting method and a chip. A microcolumn array is arranged in the sorting area of the microfluidic chip, and the cross-sectional shape of the microcolumn is peach-shaped or drop-shaped. Realize the high-throughput and high-precision sorting, capture, storage, on-demand release and collection functions of target cells, and provide a powerful research tool for subsequent testing of cells.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to a microfluidic chip for sorting and capturing rare cells, a manufacturing method and an application method thereof. Background technique [0002] Research by the World Health Organization shows that tumor metastasis causes as many as 90% of cancer deaths. If tumor metastasis can be detected and treated early, at least 30% of the deaths can be avoided. Circulating tumor cells (CTCs), as a rare type of cells in human peripheral blood, are clinically considered to be an early sign of tumor metastasis, so the detection of circulating tumor cells in blood can be used for early diagnosis of cancer. significant. The content of circulating tumor cells in human blood is extremely low, and the content in 1ml of patient blood is only 1-100. Therefore, how to accurately and efficiently remove blood cells and isolate rare circulating tumor cells on the premise of maintaining cell viability is an ...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/09
CPCC12M23/16C12M47/04C12N5/0693
Inventor 吴一辉刘永顺李高林纪元
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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